Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM291
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Product Specifications
Immunogen
Human milk fat globule membrane preparation (Uniprot: Q08431)
Localization
Cell Surface & Cytoplasmic
Marker
Breast Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11482)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for MFG-E8 Antibody (SPM291)
![Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411]](https://beta-resources.rndsystems.com/images/products/MFG-E8-Antibody-SPM291-Immunohistochemistry-Paraffin-NBP2-44411-img0004.jpg "Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411]")
Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411]
Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with MFG-E8 Antibody (SPM291)![Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411]](https://beta-resources.rndsystems.com/images/products/MFG-E8-Antibody-SPM291-Immunohistochemistry-Paraffin-NBP2-44411-img0001.jpg "Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411]")
Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411]
Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411] - Human Breast Carcinoma stained with Milk Fat Globule Monoclonal Antibody (SPM291)![Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411]](https://beta-resources.rndsystems.com/images/products/MFG-E8-Antibody-SPM291-Immunohistochemistry-Paraffin-NBP2-44411-img0002.jpg "Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411]")
Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411]
Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with MFG-E8 Antibody (SPM291).![Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411]](https://beta-resources.rndsystems.com/images/products/MFG-E8-Antibody-SPM291-Immunohistochemistry-Paraffin-NBP2-44411-img0003.jpg "Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411]")
Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411]
Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411] - Formalin-fixed, paraffin-embedded human Pancreas stained with MFG-E8 Antibody (SPM291)Applications for MFG-E8 Antibody (SPM291)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: MFG-E8
Long Name
Milk Fat Globule EGF Factor 8
Alternate Names
BA46, Breast epithelial antigen BA46, Lactahedrin, Medin, MFGE8, SED1
Gene Symbol
MFGE8
UniProt
Additional MFG-E8 Products
Product Documents for MFG-E8 Antibody (SPM291)
Product Specific Notices for MFG-E8 Antibody (SPM291)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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