MGAT1 Antibody

Novus Biologicals | Catalog # NBP1-52077

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all N-Acetylglucosaminyltransferase I/MGAT1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QVEKVRTNDR corresponding to internal region according to NP_002397.2.

Epitope

C-QVEKVRTNDR

Specificity

Reported variants represent identical protein: NP_002397.2, NP_001108090.1, NP_001108091.1, NP_001108092.1, NP_001108089.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MGAT1 Antibody

Western Blot: MGAT1 Antibody [NBP1-52077]

Western Blot: MGAT1 Antibody [NBP1-52077]

Western Blot: MGAT1 Antibody [NBP1-52077] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MGAT1 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1mg/ml) in Lane B.

Applications for MGAT1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.2 - 1 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing full-length human MGAT1 (myc and DYKDDDDK tagged), a band of approx. 55 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: N-Acetylglucosaminyltransferase I/MGAT1

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene.

Long Name

Mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase

Alternate Names

GGNT1, GlcNac-TI, GLCT1, GNT-1

Entrez Gene IDs

4245 (Human); 17308 (Mouse); 81519 (Rat)

Gene Symbol

MGAT1

UniProt

NP_002397.2 (Human)

Additional N-Acetylglucosaminyltransferase I/MGAT1 Products

Product Documents for MGAT1 Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for MGAT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

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