mGluR1 Antibody - BSA Free

Novus Biologicals | Catalog # NLS882

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Chimpanzee, Monkey, Primate

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all mGluR1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 16 amino acid peptide from C-terminus of human mGluR1.

Epitope

C-Terminus

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rabbit (81%).

Specificity

Human mGluR1, isoform alpha. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for mGluR1 Antibody - BSA Free

Immunohistochemistry-Paraffin: mGluR1 Antibody - BSA Free [NLS882]

Immunohistochemistry-Paraffin: mGluR1 Antibody - BSA Free [NLS882]

Immunohistochemistry-Paraffin: mGluR1 Antibody [NLS882] - Anti-GRM1 / MGLUR1 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: mGluR1 Antibody - BSA Free [NLS882]

Immunohistochemistry-Paraffin: mGluR1 Antibody - BSA Free [NLS882]

Immunohistochemistry-Paraffin: mGluR1 Antibody [NLS882] - Anti-GRM1 / MGLUR1 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: mGluR1 Antibody - BSA Free [NLS882]

Immunohistochemistry-Paraffin: mGluR1 Antibody - BSA Free [NLS882]

Immunohistochemistry-Paraffin: mGluR1 Antibody [NLS882] - Anti-GRM1 / MGLUR1 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Applications for mGluR1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

2 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: mGluR1

The Metabotropic Glutamate Receptor mGluR1 binds L-glutamate, the major excitatory neurotransmitter in the central nervous system, which activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. mGluR1 and mGluR5 are group I receptors that have been shown to activate phospholipase C. Alternative splice variants of the mGluR1 gene have been described, but their full-length nature has not been determined. Inflammation results in the activation of mGluR1 and mGluR5 in dorsal horn neurons of the spinal cord, leading to extracellular signalregulated kinase (ERK) activation, which is required for nociceptive plasticity and enhanced pain.

Long Name

Metabotropic Glutamate Receptor 1

Alternate Names

GPRC1A, GRM1

Entrez Gene IDs

2911 (Human)

Gene Symbol

GRM1

UniProt

Q13255

Additional mGluR1 Products

Product Documents for mGluR1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for mGluR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for mGluR1 Antibody - BSA Free

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Protocols

View specific protocols for mGluR1 Antibody - BSA Free (NLS882):

Immunohistochemistry Protocol for metabotropic Glutamate Receptor 1 Antibody (NLS882):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

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