MHC Class I Antibody (MEM-E/06) - BSA Free

Novus Biologicals | Catalog # NB500-307

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-E/06

Format

BSA Free
View all MHC Class I Primary Antibodies »

Product Specifications

Immunogen

Bacterially expressed recombinant full length protein refolded with beta2-microglobulin and peptide (Human).

Localization

Type I membrane protein.

Specificity

This antibody (clone MEM-E/06) recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. Some resultss showed that this antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7). However, others have confirmed that this antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MHC Class I Antibody (MEM-E/06) - BSA Free

Immunohistochemistry: MHC Class I Antibody (MEM-E/06) - BSA Free [NB500-307]

Immunohistochemistry: MHC Class I Antibody (MEM-E/06) - BSA Free [NB500-307]

Immunohistochemistry: MHC Class I Antibody (MEM-E/06) [NB500-307] - Staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/06).
Flow Cytometry: MHC Class I Antibody (MEM-E/06) - BSA Free [NB500-307]

Flow Cytometry: MHC Class I Antibody (MEM-E/06) - BSA Free [NB500-307]

Flow Cytometry: MHC Class I Antibody (MEM-E/06) [NB500-307] - Analysis using PE conjugate of NB500-307. A cell surface stain was performed on THP-1 cells with MHC Class I antibody (MEM-E/06) NB500-307 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at room temperature. Both antibodies were conjugated to PE.

Applications for MHC Class I Antibody (MEM-E/06) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

1:50
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MHC Class I

HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.

Alternate Names

DKFZp686P19218, EA1.2, EA2.1, HLA class I histocompatibility antigen, alpha chain E, HLA class I histocompatibility antigen, E alpha chain, HLA-6.2, HLAE, lymphocyte antigen, major histocompatibility complex, class I, E, MHC, MHC class I antigen E, MHC HLA-E alpha-1, MHC HLA-E alpha-2.1, QA1

Entrez Gene IDs

3133 (Human)

Gene Symbol

HLA-E

Additional MHC Class I Products

Product Documents for MHC Class I Antibody (MEM-E/06) - BSA Free

Certificate of Analysis

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Product Specific Notices for MHC Class I Antibody (MEM-E/06) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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