Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Block/Neutralize, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2 Clone # AP-MAB0874

Format

Azide and BSA Free
View all MHC Class II Primary Antibodies »

Product Specifications

Immunogen

Activated C57BL/6 mouse spleen cells

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Description

Novus Biologicals Rat MHC Class II Antibody (AP-MAB0874) - Azide and BSA Free (NBP1-06696) is a monoclonal antibody validated for use in IHC, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Applications for MHC Class II Antibody (AP-MAB0874) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MHC Class II

MAB0874 reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Alternate Names

class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM

Entrez Gene IDs

14998 (Mouse)

Gene Symbol

HLA-DMA

UniProt

P28078 (Mouse)

Additional MHC Class II Products

Product Documents for MHC Class II Antibody (AP-MAB0874) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MHC Class II Antibody (AP-MAB0874) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MHC Class II Antibody (AP-MAB0874) - Azide and BSA Free

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    • Q: I am searching for isotype antibody for MHC Class II Antibody (AP-MAB0874), would you please let me know the most suitable isotype?
      A: The isotype of NBP1-06696 is IgG2b. Here is a link to our rat IgG2b isotype controls.
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