Mitofusin 2 Antibody (4H10F8)

Novus Biologicals | Catalog # NBP3-15855

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4H10F8 expressed in HEK293
View all Mitofusin 2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Mitofusin 2 (O95140). MSLLFSRCNSIVTVKKNKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESATFLEDTYRNAELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mitofusin 2 Antibody (4H10F8)

Western Blot: Mitofusin 2 Antibody (4H10F8) [NBP3-15855]

Western Blot: Mitofusin 2 Antibody (4H10F8) [NBP3-15855]

Western Blot: Mitofusin 2 Antibody (4H10F8) [NBP3-15855] - Western blot analysis of extracts of various cell lines, using Mitofusin 2 antibody (NBP3-15855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Mitofusin 2 Antibody (4H10F8)

Immunocytochemistry/ Immunofluorescence: Mitofusin 2 Antibody (4H10F8) [Mitofusin 2] -

Immunocytochemistry/ Immunofluorescence: Mitofusin 2 Antibody (4H10F8) [Mitofusin 2] - Confocal imaging of paraffin-embedded Mouse brain tissue using Mitofusin 2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Applications for Mitofusin 2 Antibody (4H10F8)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Mitofusin 2

MFN2 encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified.

Alternate Names

CMT2A, CMT2A2, CPRP1, HSG, MARF, MFN2

Gene Symbol

MFN2

Additional Mitofusin 2 Products

Product Documents for Mitofusin 2 Antibody (4H10F8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Mitofusin 2 Antibody (4H10F8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators
Obesity

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