MKK3/MEK3 Antibody (8U2X3)

Name: MKK3/MEK3 Antibody (8U2X3)
Brand: Novus Biologicals
SKU: NBP3-16163
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16163

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8U2X3 expressed in HEK293

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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 150-347 of human MKK3/MEK3 (P46734). FYRKVLDKNMTIPEDILGEIAVSIVRALEHLHSKLSVIHRDVKPSNVLINKEGHVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKSDVWSLGITMIEMAILRFPYESWGTPFQQLKQVVEEPSPQLPADRFSPEFVDFTAQCLRKNPAERMSYLELMEHPFFTLHKTKKTDIAAFVKEILGEDS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MKK3/MEK3 Antibody (8U2X3)

Western Blot: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163]

Western Blot: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163] - Western blot analysis of extracts of various cell lines, using MKK3/MEK3 Rabbit mAb (NBP3-16163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunocytochemistry/ Immunofluorescence: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163] -

Immunocytochemistry/ Immunofluorescence: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163] - Confocal imaging of C6 cells using MKK3/MEK3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Immunocytochemistry/ Immunofluorescence: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163] -

Immunocytochemistry/ Immunofluorescence: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163] - Confocal imaging of NIH/3T3 cells using MKK3/MEK3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for MKK3/MEK3 Antibody (8U2X3)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MKK3

Mitogen-acitvated protein kinase kinase 3 (MKK3) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). MKK3, closely related to MKK6, efficiently phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines and environmental stress but not JNK or ERK (3). MKK3 is activated by mitogenic and environ-mental stress stimulus as well as by insulin. MKK3 is necessary for the expression of glucose transporter and participates in the MAP kinase- mediated signaling cascade (4).

Long Name

Mitogen-activated Protein Kinase Kinase 3

Alternate Names

MAP2K3, MEK3, PRKMK3

Gene Symbol

MAP2K3

Additional MKK3 Products

Product Documents for MKK3/MEK3 Antibody (8U2X3)

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Certificate of Analysis

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Product Specific Notices for MKK3/MEK3 Antibody (8U2X3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.