MLF1 Interacting Protein Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-45908

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MLF1 Interacting Protein Primary Antibodies »

Product Specifications

Immunogen

MLF1 interacting protein. (Uniprot# Q71F23)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MLF1 Interacting Protein Antibody - BSA Free

MLF1 Interacting Protein Antibody - BSA Free

Immunohistochemistry: MLF1 Interacting Protein Antibody - BSA Free [NBP3-45908] -

Immunohistochemistry: MLF1 Interacting Protein Antibody - BSA Free [NBP3-45908] - Immunohistochemistry of paraffin-embedded human testis tissue slide using (MLF1 Interacting Protein Antibody) at dilution of 1:50
MLF1 Interacting Protein Antibody - BSA Free

Western Blot: MLF1 Interacting Protein Antibody - BSA Free [NBP3-45908] -

Western Blot: MLF1 Interacting Protein Antibody - BSA Free [NBP3-45908] - HepG2 cells were subjected to SDS PAGE followed by western blot with (MLF1 Interacting Protein antibody) at dilution of 1:500

Applications for MLF1 Interacting Protein Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:20-1:200

Western Blot

1:500-1:2000
Application Notes
Theoretical Molecular Weight is 46-48 kDa

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% Glycerol, pH (7.3)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MLF1 Interacting Protein

Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/ anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.

Alternate Names

CENP-50, CENP-U, CENPU50, CENPUMLF1-interacting protein, centromere protein of 50 kDa, centromere protein U, FLJ23468, ICEN24, Interphase centromere complex protein 24, KLIP1CENP50, KSHV latent nuclear antigen interacting protein 1, KSHV latent nuclear antigen-interacting protein 1, MLF1 interacting protein, PBIP1, Polo-box-interacting protein 1

Gene Symbol

CENPU

Additional MLF1 Interacting Protein Products

Product Documents for MLF1 Interacting Protein Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MLF1 Interacting Protein Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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