MLL3 Antibody (PSH02-11)

Novus Biologicals | Catalog # NBP3-32507

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH02-11 expressed in HEK293
View all MLL3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within human MLL3 aa 3,251-3,550 / 4,911. (Uniprot: Q8NEZ4)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

541 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MLL3 Antibody (PSH02-11)

MLL3 Antibody (PSH02-11)

Western Blot: MLL3 Antibody (PSH02-11) [NBP3-32507]

Western Blot: MLL3 Antibody (PSH02-11) [NBP3-32507] - Western blot analysis of MLL3 on different lysates with Rabbit anti-MLL3 antibody (NBP3-32507) at 1/1,000 dilution.

Lane 1: U-2 OS cell lysate (20 ug/Lane)
Lane 2: MCF7 cell lysate (20 ug/Lane)
Lane 3: HCT 116 cell lysate (20 ug/Lane)
Lane 4: Rat skeletal muscle tissue lysate (40 ug/Lane)

Predicted band size: 541 kDa
Observed band size: 541 kDa

Exposure time: 1 minute 36 seconds;

3-8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32507) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for MLL3 Antibody (PSH02-11)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MLL3

The MLL3 gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a nuclear protein withan AT hook DNA-binding domain, a DHHC-type zinc finger, six PHD-type zinc fingers, a SET domain, a post-SET domain anda RING-type zinc f

Alternate Names

ALR-like protein, DKFZp686C08112, EC 2.1.1.43, FLJ12625, HALRMGC119851, histone-lysine N-methyltransferase MLL3, histone-lysine N-methyltransferase, H3 lysine-4 specific, Homologous to ALR protein, KIAA1506FLJ38309, KMT2C, KMT2CMGC119852, Lysine N-methyltransferase 2C, MGC119853, myeloid/lymphoid or mixed-lineage leukemia 3, Myeloid/lymphoid or mixed-lineage leukemia protein 3

Gene Symbol

KMT2C

Additional MLL3 Products

Product Documents for MLL3 Antibody (PSH02-11)

Certificate of Analysis

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Product Specific Notices for MLL3 Antibody (PSH02-11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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