Mouse MMP-1 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-06885

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

75 pg/mL

Assay Range

125 - 8000 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Specificity

This kit recognizes Mouse MMP-1 in samples. No significant cross-reactivity or interference between Mouse MMP-1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV 5.67

Inter-Assay Precision (Precision between assays) %CV 4.63

Recovery for Mouse MMP-1 ELISA Kit (Colorimetric)

Recovery

87 - 107%

Linearity

Mouse MMP-1 ELISA Kit (Colorimetric)

Scientific Data Images for Mouse MMP-1 ELISA Kit (Colorimetric)

Mouse MMP-1 ELISA Kit (Colorimetric)

ELISA: Mouse MMP-1 ELISA Kit (Colorimetric) [NBP3-06885] -

ELISA: Mouse MMP-1 ELISA Kit (Colorimetric) [NBP3-06885] - Standard Curve Reference

Kit Contents for Mouse MMP-1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MMP-1

Human interstitial collagenase (matrix metalloproteinase-1, MMP-1), an enzyme whose only known physiologic substrate has heretofore been believed to be the extracellular matrix molecule, collagen. Data indicate that matrix metalloproteinase-1 displays an expanded substrate repertoire that supports the existence of a new interface between connective tissue turnover and serine proteinase inhibitors (1). It has been shown that the MMP-1 functions as a protease agonist of Protease-activated receptors (PAR1) cleaving the receptor at the proper site to generate PAR1-dependent Ca2+ signals and migration. These results demonstrate that MMP-1 in the stromal-tumor microenvironment can alter the behavior of cancer cells through PAR1 to promote cell migration and invasion (2). It has also been suggested that increased levels of MMP-1 due to tobacco smoking plays a major role in the aging process of skin since MMP-1 degrades collagen, which accounts for at least 70% of the dry weight of dermis. Significantly more MMP-1 has been detected in the skin of smokers than non-smokers whereas no difference was seen for the tissue inhibitor of metalloproteinases 1 (3).

Long Name

Matrix Metalloproteinase 1

Alternate Names

MMP1

Gene Symbol

MMP1

Additional MMP-1 Products

Product Documents for Mouse MMP-1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Mouse MMP-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Mouse MMP-1 ELISA Kit (Colorimetric)

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