Key Product Details

Species Reactivity

Virus

Applications

Western Blot, ELISA, Lateral Flow Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 14

Format

Azide and BSA Free
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Product Specifications

Immunogen

Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Monkeypox Virus E8L (RefSeq#: QNP13700.1; Met1-Thr275)

Reactivity Notes

Monkeypox Virus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Monkeypox Virus E8L Antibody (14) - Azide and BSA Free (NBP3-30751) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Monkeypox Virus E8L Antibody (14) - Azide and BSA Free

Monkeypox Virus E8L Antibody (14) - Azide and BSA Free

Western Blot: Monkeypox Virus E8L Antibody (14) [NBP3-30751] -

Western Blot: Monkeypox Virus E8L Antibody (14) [NBP3-30751] - NBP3-30751 at 1:10000 dilution. Lane A: Monkeypox Virus (MPXV) Envelope protein E8L Protein (10ng) Secondary Goat Anti-Mouse IgG (H+L) [HRP] at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions.

Applications for Monkeypox Virus E8L Antibody (14) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:1000-1:2000

Lateral Flow Assay

1 mg/ml

Western Blot

1:2000-1:10000
Application Notes
In Lateral Flow Assay, NBP3-30752 (1-2 mg/ml recommended dilution) conjugated with colloidal gold particles and paired with NBP3-30751 as the Capture Antibody to detect recombinant Monkeypox Virus E8L Protein, the sensitivity is 100 pg/mL.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: E8L

Long Name

Cell Surface-Binding Protein (MPXV-E8L)

Alternate Names

MPXV-E8L, OPG120

Entrez Gene IDs

QNP13700.1 (Virus)

Additional E8L Products

Product Documents for Monkeypox Virus E8L Antibody (14) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Monkeypox Virus E8L Antibody (14) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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