Monocyte/Macrophage Antibody (ER-HR3)

Novus Biologicals | Catalog # NBP3-11247

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2C Clone # ER-HR3
View all Monocyte/Macrophage Primary Antibodies »

Product Specifications

Immunogen

The monoclonal antibody ER-HR3 is reactive to the majority of mouse monocytes and a subset of mature resident macrophages, especially those located in hemopoietic organs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2C

Applications for Monocyte/Macrophage Antibody (ER-HR3)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Flow Cytometry and Immunohistochemistry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Monocyte/Macrophage

The monoclonal antibody ER-HR3 is reactive to the majority of mouse monocytes and a subset of mature resident macrophages, especially those located in hemopoietic organs. Whereas all other leukocytes are ER-HR3 negative, up to 70% of circulating monocytes stain positive with this monoclonal antibody. Furthermore, about 30% of bone marrow cells express the antigen. Other ER-HR3 positive macrophages can be found in the splenic red pulp, in the mesenteric lymphoid paracortex, interfollicular areas of Peyer's patches and bone marrow. Few ER-HR3 positive cells are observed in the thymic cortex and the connective tissues of the gastro-intestinal tract, the dermis and the renal medulla. Epidermal Langerhans cells also express the antigen. The monoclonal antibody ER-HR3 can be used to identify and localize a very distinct mature tissue macrophage subpopulation found in various mouse organs and its distribution pattern is unique compared to that from other macrophage-specific antibodies. The antibody is especially suitable for ontogenic studies because ER-HR3 positive macrophages are closely related to hemopoietic islands, especially at erythropoietic sites.

Alternate Names

macrophage, macrophages, monocyte, monocytes

Additional Monocyte/Macrophage Products

Product Documents for Monocyte/Macrophage Antibody (ER-HR3)

Certificate of Analysis

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Product Specific Notices for Monocyte/Macrophage Antibody (ER-HR3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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