Mouse 5'-Nucleotidase/CD73 Antibody

Catalog # Availability Size / Price Qty
MAB44881
MAB44881-SP
Product Details
Citations (2)
FAQs
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Mouse 5'-Nucleotidase/CD73 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse 5'‑Nucleotidase/CD73 in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 496424
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse 5'‑Nucleotidase/CD73
Trp29-Lys549
Accession # Q61503
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse 5'-Nucleotidase/CD73 (Catalog # 4488-EN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5'-Nucleotidase/CD73

CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3). The amino acid sequence of mouse CD73 is 94% and 87% identical to that of rat and human CD73, respectively.

References
  1. Resta, R. et al. (1993) Gene 133:171.
  2. Resta, R. et al. (1998) Immunol. Rev. 161:95.
  3. Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.
Entrez Gene IDs
4907 (Human); 23959 (Mouse); 58813 (Rat); 102114976 (Cynomolgus Monkey)
Alternate Names
5' nucleotidase (CD73); 5'-NT; 5-NT; 5'-Nucleotidase; 5'-nucleotidase, ecto (CD73); CD73 antigen; CD73; E5NT; EC 3.1.3.5; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase; NT5E; NTE; Purine 5-Prime-Nucleotidase

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Citations for Mouse 5'-Nucleotidase/CD73 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Role of P2XReceptor in Mouse Voiding Function
    Authors: W Yu, WG Hill, SC Robson, ML Zeidel
    Sci Rep, 2018;8(1):1838.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Expression and distribution of ectonucleotidases in mouse urinary bladder.
    Authors: Yu W, Robson SC, Hill WG
    PLoS ONE, 2011;6(4):e18704.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Secondary Antibodies

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