Mouse BACE-2 Ectodomain Alexa Fluor® 488-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: BACE-2

BACE-2 is an aspartic protease that shares sequence similarity with BACE-1, the major beta -secretase for generation of A beta peptide in neurons (1). However, BACE-2 differs from BACE-1 in several aspects including pro-enzyme activation, substrate binding sites, transcriptional regulation, and function in A beta peptide production (2‑4). The domain structure of mouse BACE-2 consists of a signal peptide (aa 1‑19), pro (aa 20‑62) and a mature chain (aa 63‑514) that consists of a transmembrane domain and a cytoplasmic tail. The C-terminal region (aa 463‑514) was replaced by a His tag in the recombinant mouse BACE-2 that also contained a His residue at the position of Tyr357. The purified recombinant mouse BACE-2 started at Ala63, the same residue shown at the N-terminus of the human active BACE-2 (2).

Product Datasheets

Related Research Areas

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