Mouse BACH1 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF5777R Datasheet / COA / SDS

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AF5777R-100UG

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Mouse BACH1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects endogenous mouse BACH1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse BACH1
Ser133-Gly513
Accession # P97302

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BACH1

BACH1 (BTB and CNC homolog 1; also basic leucine zipper transcription factor 1) is a ubiquitously expressed member of the Bach family of transcription factors. Although its predicted MW is 82 kDa, it runs anomalously at 110 kDa in SDS-PAGE. BACH1 forms noncovalent homodimers, and heterodimers with Maf oncoproteins and p53-related proteins. It apparently serves as an architectural component for gene regulatory protein complexes. Mouse BACH1 is 739 amino acids (aa) in length. It contains a protein-interaction BTB domain (aa 24‑127), a DNA-binding motif (aa 565‑580), and a Leu-zipper domain (aa 588‑610). This molecule should not be confused with Bach1/Fancj/Brip1 helicase. Over aa 133‑513, mouse BACH1 shares 75% and 88% aa identity with human and rat BACH1, respectively.

Long Name

BTB and CNC homolog 1

Entrez Gene IDs

571 (Human); 12013 (Mouse); 304127 (Rat)

Alternate Names

BACH1; basic region leucine zipper transcriptional regulator BACH1; BTB and CNC homolog 1; BTB and CNC homology 1, basic leucine zipper transcription factor 1; BTB; HA2303; transcription regulator protein BACH1; transcription regulator protein10BACH-1