Mouse BACH1 Antibody
Mouse BACH1 Antibody Summary
Accession # P97302
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse BACH1 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse BACH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5777) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BACH1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
BACH1 (BTB and CNC homolog 1; also basic leucine zipper transcription factor 1) is a ubiquitously expressed member of the Bach family of transcription factors. Although its predicted MW is 82 kDa, it runs anomalously at 110 kDa in SDS-PAGE. BACH1 forms noncovalent homodimers, and heterodimers with Maf oncoproteins and p53-related proteins. It apparently serves as an architectural component for gene regulatory protein complexes. Mouse BACH1 is 739 amino acids (aa) in length. It contains a protein-interaction BTB domain (aa 24‑127), a DNA-binding motif (aa 565‑580), and a Leu-zipper domain (aa 588‑610). This molecule should not be confused with Bach1/Fancj/Brip1 helicase. Over aa 133‑513, mouse BACH1 shares 75% and 88% aa identity with human and rat BACH1, respectively.
Citations for Mouse BACH1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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PGRMC2 is an intracellular haem chaperone critical for adipocyte function
Authors: A Galmozzi, BP Kok, AS Kim, JR Montenegro, JY Lee, R Spreafico, S Mosure, V Albert, R Cintron-Co, C Godio, WR Webb, B Conti, LA Solt, D Kojetin, CG Parker, JJ Peluso, JK Pru, G Siuzdak, BF Cravatt, E Saez
Sample Types: Tissue Lysates
Applications: Western Blot
Specific sequences of infectious challenge lead to secondary hemophagocytic lymphohistiocytosis-like disease in mice
Authors: A Wang, SD Pope, JS Weinstein, S Yu, C Zhang, CJ Booth, R Medzhitov
Proc. Natl. Acad. Sci. U.S.A., 2019-01-23;0(0):.
Sample Types: Cell Lysates
Applications: Western Blot
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