Detects mouse BAFF in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) 4-1BB Ligand, rmEDA, rmFas Ligand, rmOX40 Ligand, rmTNF-alpha, rmTRANCE, rmTWEAK, recombinant human (rh) APRIL, rhBAFF, rhEDA-A2, rhGITR Ligand, rhLIGHT, rhLymphotoxin alpha 1/ beta 2, rhLymphotoxin alpha 2/ beta 1, rhTNF-alpha, rhTRAIL, rhVEGI, recombinant canine, cotton rat, equine, feline, porcine, or rat TNF-alpha.
Monoclonal Rat IgG2A Clone # 121808
Protein A or G purified from hybridoma culture supernatant
Detection of BAFF/BLyS/TNFSF13B in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse BAFF/BLyS/TNFSF13B PE‑conjugated Monoclonal Antibody (Catalog # IC1357P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
BAFF (also known as TALL-1, BLyS, THANK) is a 34-36 kDa type II transmembrane glycoprotein belonging to the BAFF family of the TNF superfamily, and has been designated as TNF superfamily member 13B (TNFSF13B). Mouse BAFF is a 309 amino acid (aa) protein consisting of a 248 aa extracellular domain, a 21 aa transmembrane region and a 45 aa cytoplasmic tail. BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain. Mouse BAFF may be shed from the cell surface by proteolytic cleavage between R126 and Ala 127 to yield a soluble form of the protein detectable in serum. Within the TNF superfamily, BAFF shares the highest homology (48%) with APRIL, the only other member of the BAFF family of molecules. BAFF and APRIL both bind to BCMA and TACI, while BAFF also binds specifically to BAFF receptor (BAFF R, also known as BR3 or TNFSFR13C), which represents the principal BAFF receptor. All three receptors are type III transmembrane proteins that are expressed on B cells, monocytes, osteoclasts, activated T cells, adipocytes, keratinocytes and microglia. BAFF and APRIL can form active heteromers that bind TACI. BAFF is expressed by monocytes, osteoclasts, astrocytes, neutrophils, follicular T helper cells, dendritic cells and specialized fibroblasts. Its expression in resting monocytes is upregulated by IFN-alpha, IFN-beta, LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response. BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells. Recent reports also show a role for BAFF in osteoclast formation. Over aa 127-309, mouse BAFF shares 93 and 72% aa sequence identity with rat and human BAFF, respectively.
B cell Activating Factor
Entrez Gene IDs:
10673 (Human); 24099 (Mouse); 498666 (Rat)
ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4
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