Mouse C1q R1/CD93 Quantikine ELISA Kit

    
Datasheet / CoA / SDS
Product Details
Assay Procedure
FAQs
Supplemental Products
Reviews
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Cell Lysates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    11.4 pg/mL
  • Assay Range
    78.1 - 5,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse C1q R1
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse C1q R1/CD93 Immunoassay is a 3.5 hour solid phase ELISA designed to measure mouse C1q R1 levels in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant mouse C1q R1 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse C1q R1 accurately. Results obtained using natural mouse C1q R1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse C1q R1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 22 22 22
Mean 248 704 2033 256 692 2085
Standard Deviation 2.81 13.2 29.2 19.2 33.2 116
CV% 1.1 1.9 1.4 7.5 4.8 5.6

Recovery

The recovery of mouse C1q R1 spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=8) 95 84-105
Cell Lysates (n=4) 110 102-116
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse C1q R1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: C1q R1/CD93
C1q R1 is also known as C1q Rp, collectin receptor, and AA4 antigen. C1q R1 mediates enhanced phagocytosis by monocytes and macrophages upon interaction with soluble defense collagens including C1q, MBL, and SP-A. It is a type I transmembrane glycoprotein expressed on endothelial cells, hematopoietic progenitor cells, platelets, and cells of myeloid origin. C1q R1 has also been identified as a hematopoietic stem cell marker.
  • Long Name:
    Complement Component C1q Receptor
  • Entrez Gene IDs:
    22918 (Human); 17064 (Mouse); 84398 (Rat)
  • Alternate Names:
    AA4 Antigen; C1q R1; C1q receptor 1; C1q Rp; C1q/MBL/SPA receptor; C1qR(P); C1qR1; C1qRp; CD93 antigenC1qR; CD93 molecule; CD93; CDw93C1QR1; Collectin Receptor; Complement component 1 q subcomponent receptor 1; complement component 1, q subcomponent, receptor 1; complement component C1q receptor; dJ737E23.1; ECSM3; Ly68; Matrix-remodeling-associated protein 4; matrix-remodelling associated 4; MXRA4
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs
Supplemental ELISA Products
Description Application Cat# Citations Images  

Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse C1q R1/CD93 Quantikine ELISA Kit?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
R&D Systems Guarantee