Mouse Carbonic Anhydrase XII/CA12 Alexa Fluor™ Plus 555‑conjugated Antibody

R&D Systems | Catalog # AF2345AFP555

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunoprecipitation

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Polyclonal Goat IgG
View all Carbonic Anhydrase XII/CA12 Primary Antibodies »

Product Specifications

Specificity

Detects mouse Carbonic Anhydrase XII/CA12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human CA12 is observed and less than 5% cross-reactivity with recombinant human CA1, rhCA2, rhCA3, rhCA8, recombinant mouse (rm) CA4, and rmCA9 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Carbonic Anhydrase XII/CA12

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Mouse CA12 is predicted to be a type I membrane enzyme (2, 3). The secreted, purified rmCA12 corresponds to the extracellular domain and has both CA activity and esterase activity. To our knowledge, this is the first description of purified rmCA12 with enzymatic activity.

References

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
  3. Okazaki, Y. et al. (2002) Nature 420:563.

Alternate Names

CA12

Entrez Gene IDs

771 (Human); 76459 (Mouse)

Gene Symbol

CA12

UniProt

Q8CI85

Additional Carbonic Anhydrase XII/CA12 Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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