Mouse Carbonic Anhydrase XII/CA12 Antibody

Catalog # Availability Size / Price Qty
AF2345
AF2345-SP
Product Details
Citations (2)
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Mouse Carbonic Anhydrase XII/CA12 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Carbonic Anhydrase XII/CA12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human CA12 is observed and less than 5% cross-reactivity with recombinant human CA1, rhCA2, rhCA3, rhCA8, recombinant mouse (rm) CA4, and rmCA9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase XII/CA12
Ala25-Ser301
Accession # Q8CI85
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Carbonic Anhydrase XII (Catalog # 2345-CA)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Carbonic Anhydrase XII (Catalog # 2345-CA), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase XII/CA12

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Mouse CA12 is predicted to be a type I membrane enzyme (2, 3). The secreted, purified rmCA12 corresponds to the extracellular domain and has both CA activity and esterase activity. To our knowledge, this is the first description of purified rmCA12 with enzymatic activity.

References
  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
  3. Okazaki, Y. et al. (2002) Nature 420:563.
Entrez Gene IDs
771 (Human); 76459 (Mouse)
Alternate Names
CA12; Carbonate dehydratase XII; carbonic anhydrase 12; Carbonic Anhydrase XII; carbonic anhydrase XIICAXII; carbonic dehydratase; CA-XII; EC 4.2.1.1; FLJ20151; HsT18816; Tumor antigen HOM-RCC-3.1.3

Product Datasheets

Citations for Mouse Carbonic Anhydrase XII/CA12 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CO2 chemosensing in rat oesophagus.
    Authors: Akiba Y, Mizumori M, Kuo M, Ham M, Guth PH, Engel E, Kaunitz JD
    Gut, 2008;57(12):1654-64.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  2. Carbonic anhydrases and mucosal vanilloid receptors help mediate the hyperemic response to luminal CO2 in rat duodenum.
    Authors: Akiba Y, Ghayouri S, Takeuchi T, Mizumori M, Guth PH, Engel E, Swenson ER, Kaunitz JD
    Gastroenterology, 2006;131(1):142-52.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC

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