Mouse CCL21/6Ckine Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB4572-100
MAB4572-SP
Chemotaxis Induced by CCL21/6Ckine and Neutralization by Mouse CCL21/6Ckine Antibody.
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Mouse CCL21/6Ckine Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL21/6Ckine in direct ELISAs.
Source
Recombinant Monoclonal Rat IgG2A Clone # 709408R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant mouse CCL21/6Ckine
Accession # P84444
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize CCL21/6Ckine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR7. The Neutralization Dose (ND50) is typically 0.5-3 µg/mL in the presence of 50 ng/mL Recombinant Mouse CCL21/6Ckine.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Chemotaxis Induced by CCL21/6Ckine and Neutralization by Mouse CCL21/6Ckine Antibody. View Larger

Chemotaxis Induced by CCL21/6Ckine and Neutralization by Mouse CCL21/6Ckine Antibody. Recombinant Mouse CCL21/6Ckine (Catalog # 457-6C) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL21/6Ckine (50 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL21/6Ckine Monoclonal Antibody (Catalog # MAB4572). The ND50 is typically 0.5-3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL21/6Ckine

6Ckine is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (secondary lymphoid-tissue chemokine), CCL21 and Exodus-2, shows 21‑33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxyl-terminal domain. Human 6Ckine cDNA encodes a 134 amino acid highly basic precursor protein with a 23 amino acid residue signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Human and mouse 6Ckine share 86% amino acid sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. Unlike most CC chemokines, 6Ckine is not chemotactic for monocytes. Recombinant mouse 6Ckine is chemotactic in vitro for thymocytes and activated T cells. Recombinant human 6Ckine has been shown to be chemotactic for some human T cell lines, resting PBL, and cultured T cells expanded with PHA and IL-2. 6Ckine has also been reported to inhibit hemopoietic progenitor colony formation in a dose-dependent manner. 6Ckine acts via the CC receptor CCR7 on T cells and B cells.

References
  1. Hedrick, J.A. and A. Zlotnik (1997) J. Immunol. 159:1589.
  2. Hromas, R. et al. (1997) J. Immunol. 159:2554.
  3. Nagira, M. et al. (1997) J. Biol. Chem. 272:19518.
Entrez Gene IDs
6366 (Human); 18829 (Mouse); 298006 (Rat)
Alternate Names
6Ckine; 6CkineSmall-inducible cytokine A21; Beta-chemokine exodus-2; CCL21; chemokine (C-C motif) ligand 21; CKb9; exodus-2; member 21; SCYA21; SCYA21MGC34555; secondary lymphoid tissue chemokine; SLC; SLCSecondary lymphoid-tissue chemokine; TCA-4

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