Mouse CCRL2/LCCR APC-conjugated Antibody

Catalog #: FAB5519A Datasheet / COA / SDS

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FAB5519A

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Detection of CCRL2/LCCR in J774A.1 Mouse Cell Line by Flow Cytometry.

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Mouse CCRL2/LCCR APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCRL2/LCCR in direct ELISAs.

Source

Monoclonal Rat IgG₁ Clone # 498321

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

RBL-1 rat basophilic leukemia cell line transfected with mouse CCRL2/LCCR
Accession # O35457

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCRL2/LCCR in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse CCRL2/LCCR APC-conjugated Monoclonal Antibody (Catalog # FAB5519A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCRL2/LCCR

Lipopolysaccharide-inducible CC chemokine receptor (LCCR), also known as CCRL2, is a 7TM chemokine receptor-like protein that is expressed on macrophages, glial cells, and mast cells at inflammatory sites. LCCR functions as a receptor for Chemerin, CCL2, 5, 7, and 8. Mouse LCCR shares 49% and 70% aa sequence identity with human and rat LCCR, respectively.

Long Name

CC Chemokine Receptor-like 2

Entrez Gene IDs

54199 (Mouse)

Alternate Names

ACKR5;C-C chemokine receptor-like 2;CKRX;CRAM;CRAM-A;CRAM-B;HCR; CCRL2/LCCR; LCCR