Mouse CCRL2/LCCR APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5519A
Detection of CCRL2/LCCR in J774A.1 Mouse Cell Line by Flow Cytometry.
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Mouse CCRL2/LCCR APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCRL2/LCCR in direct ELISAs.
Source
Monoclonal Rat IgG1 Clone # 498321
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
RBL-1 rat basophilic leukemia cell line transfected with mouse CCRL2/LCCR
Accession # O35457
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CCRL2/LCCR antibody in J774A.1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of CCRL2/LCCR in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse CCRL2/LCCR APC-conjugated Monoclonal Antibody (Catalog # FAB5519A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCRL2/LCCR

Lipopolysaccharide-inducible CC chemokine receptor (LCCR), also known as CCRL2, is a 7TM  chemokine receptor-like protein that is expressed on macrophages, glial cells, and mast cells at inflammatory sites. LCCR functions as a receptor for Chemerin, CCL2, 5, 7, and 8. Mouse LCCR shares 49% and 70% aa sequence identity with human and rat LCCR, respectively.

Long Name
CC Chemokine Receptor-like 2
Entrez Gene IDs
54199 (Mouse)
Alternate Names
CCRL2/LCCR; LCCR

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