Mouse CCRL2/LCCR Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCRL2/LCCR in direct ELISAs.
  • Source
    Monoclonal Rat IgG1 Clone # 498321
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    RBL-1 rat basophilic leukemia cell line transfected with mouse CCRL2/LCCR
    Accession # O35457
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCRL2/L‑CCR in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCRL2/L‑CCR Monoclonal Antibody (Catalog # MAB5519, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Immunocytochemistry
CCRL2/L‑CCR/CRAM‑A/B in RAW 264.7 Mouse Cell Line. CCRL2/L‑CCR/CRAM‑A/B was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse CCRL2/L‑CCR/CRAM‑A/B Monoclonal Antibody (Catalog # MAB5519) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCRL2/LCCR
Lipopolysaccharide-inducible CC chemokine receptor (LCCR), also known as CCRL2, is a 7TM  chemokine receptor-like protein that is expressed on macrophages, glial cells, and mast cells at inflammatory sites. LCCR functions as a receptor for Chemerin, CCL2, 5, 7, and 8. Mouse LCCR shares 49% and 70% aa sequence identity with human and rat LCCR, respectively.
  • Long Name:
    CC Chemokine Receptor-like 2
  • Entrez Gene IDs:
    54199 (Mouse)
  • Alternate Names:
    CCRL2/LCCR; LCCR
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