Mouse CD177 PE-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB8186P-025
Detection of CD177 in Mouse Bone Marrow Cells by Flow Cytometry.
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Mouse CD177 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD177 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1171A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant mouse CD177
Accession # Q8R2S8
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD177 in Mouse Bone Marrow Cells by Flow Cytometry. View Larger

Detection of CD177 in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse Gr‑1/Ly‑6G APC‑conjugated Monoclonal Antibody (Catalog # FAB1037A) and either (A) Rabbit Anti-Mouse CD177 PE‑conjugated Monoclonal Antibody (Catalog # FAB8186P) or (B) Normal Rabbit IgG Phycoerythrin Control (Catalog # IC105P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD177

CD177 is a member of the uPAR/CD59/Ly6 superfamily (1). Mature mouse CD177 is a 796 amino acid (aa) protein that contains four uPAR/Ly6 domains, while human CD177 contains only two. Within common regions, mouse CD177 shares 55% and 77% aa sequence identity with human and rat CD177, respectively. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (2-4). It is nearly absent from neutrophils from paroxysmal nocturnal hemoglobinurea patients who are deficient in the ability to synthesize GPI linkages (4, 5). It is up-regulated on granulocytes from polycythemia vera and thalassemia patients (6, 7). CD177 binds to PECAM-1 on vascular endothelial cells, an interaction which mediates neutrophil adhesion to the vascular wall and neutrophil transmigration (8). It associates in cis with the Integrin MAC-1 (CD11b/CD18) (9). CD177 also associates in cis with Proteinase 3 (PR3) and is required for cell surface PR3 expression (9-11). PR3 is normally found in intracellular vesicles, but once at the cell surface it can serve as an autoimmune target for anti-neutrophil cytoplasmic antibodies (ANCA) (12). The ANCA targeting of CD177-PR3 complexes triggers neutrophil activation and vasculitis (9, 12).

References
  1. Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
  2. Skubitz, K.M. et al. (1991) J. Leukoc. Biol. 49:163.
  3. Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
  4. Klippel, S. et al. (2002) Blood 100:2441.
  5. Boccuni, P. et al. (2000) Crit. Rev. Oncol. Hematol. 33:25.
  6. Temerinac, S. et al. (2000) Blood 95:2569.
  7. Zoi, K. et al. (2008) Brit. J. Haematol. 141:100.
  8. Sachs, U.J.H. et al. (2007) J. Biol. Chem. 282:23603.
  9. Jerke, U. et al. (2011) J. Biol. Chem. 286:7070.
  10. von Vietinghoff, S. et al. (2007) Blood 109:4487.
  11. Kuckleburg, C.J. et al. (2012) J. Immunol. 188:2419.
  12. van Timmeren, M.M. and P. Heeringa (2012) Curr. Opin. Rheumatol. 24:8.
Long Name
Polycythemia Rubra Vera Protein 1
Entrez Gene IDs
57126 (Human); 68891 (Mouse)
Alternate Names
CD177 antigenNB1 GP; CD177 molecule; CD177; HNA-2a; HNA2Acell surface receptor; Human neutrophil alloantigen 2a; NB1 GP; NB1PRV-1; polycythemia rubra vera 1; Polycythemia rubra vera protein 1; PRV1; PRV1NB1 glycoprotein

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