Mouse Gr-1/Ly-6G APC-conjugated Antibody

(2 citations)   
  • Species Reactivity
  • Specificity
    Detects mouse Gr-1/Ly-6G. Weak cross-reactivity with Ly-6C is observed.
  • Source
    Monoclonal Rat IgG2B Clone # RB6-8C5
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    The immunogen for this antibody is Ly-6G (Gr-1, Gr1).
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Gr‑1/Ly‑6G in B220- Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse Gr‑1/Ly‑6G APC‑conjugated Monoclonal Antibody (Catalog # FAB1037A) or (B) Rat IgG2B Allophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Ly-6G/Ly-6C (Gr-1)
The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).
  • References:
    1. Spangrude, G.J. et al. (1988) Science 241:58.
    2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
    3. Lewinsohn, D.M. et al.(1987) J. Immunol. 147:22.
    4. Lagasse, E. and I.L. Weissman (1996) J. Immunol. Methods 197:139.
  • Long Name:
    A Myeloid Differentiation Antigen
  • Entrez Gene IDs:
    546644 (Mouse)
  • Alternate Names:
    Gr-1; Ly-6C; Ly6G; Ly-6G
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. MicroRNA126 contributes to granulocyte colony-stimulating factor-induced hematopoietic progenitor cell mobilization by reducing the expression of vascular cell adhesion molecule 1.
    Authors: Salvucci O, Jiang K, Gasperini P
    Haematologica, 2012;97(6):818-26.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Differential IL-23 requirement for IL-22 and IL-17A production during innate immunity against Salmonella enterica serovar Enteritidis.
    Authors: Siegemund S, Schutze N, Schulz S, Wolk K, Nasilowska K, Straubinger RK, Sabat R, Alber G
    Int. Immunol., 2009;21(5):555-65.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B APC‑conjugated Isotype Control

Ctrl IC013A 3  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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