Mouse CD177 Alexa Fluor® 700-conjugated Antibody

Recombinant Monoclonal Antibody..
  • Species Reactivity
  • Specificity
    Detects mouse CD177 in direct ELISAs.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1171A
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Human embryonic kidney cell line HEK293-derived recombinant mouse CD177
    Accession # Q8R2S8
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD177 in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse Gr‑1/Ly‑6G Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1037F) and either (A) Rabbit Anti-Mouse CD177 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB8186N) or (B) Normal Rabbit IgG Alexa Fluor 700 Control (Catalog # IC105N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD177
CD177 is a member of the uPAR/CD59/Ly6 superfamily (1). Mature mouse CD177 is a 796 amino acid (aa) protein that contains four uPAR/Ly6 domains, while human CD177 contains only two. Within common regions, mouse CD177 shares 55% and 77% aa sequence identity with human and rat CD177, respectively. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (2-4). It is nearly absent from neutrophils from paroxysmal nocturnal hemoglobinurea patients who are deficient in the ability to synthesize GPI linkages (4, 5). It is up-regulated on granulocytes from polycythemia vera and thalassemia patients (6, 7). CD177 binds to PECAM-1 on vascular endothelial cells, an interaction which mediates neutrophil adhesion to the vascular wall and neutrophil transmigration (8). It associates in cis with the Integrin MAC-1 (CD11b/CD18) (9). CD177 also associates in cis with Proteinase 3 (PR3) and is required for cell surface PR3 expression (9-11). PR3 is normally found in intracellular vesicles, but once at the cell surface it can serve as an autoimmune target for anti-neutrophil cytoplasmic antibodies (ANCA) (12). The ANCA targeting of CD177-PR3 complexes triggers neutrophil activation and vasculitis (9, 12).
  • References:
    1. Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
    2. Skubitz, K.M. et al. (1991) J. Leukoc. Biol. 49:163.
    3. Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
    4. Klippel, S. et al. (2002) Blood 100:2441.
    5. Boccuni, P. et al. (2000) Crit. Rev. Oncol. Hematol. 33:25.
    6. Temerinac, S. et al. (2000) Blood 95:2569.
    7. Zoi, K. et al. (2008) Brit. J. Haematol. 141:100.
    8. Sachs, U.J.H. et al. (2007) J. Biol. Chem. 282:23603.
    9. Jerke, U. et al. (2011) J. Biol. Chem. 286:7070.
    10. von Vietinghoff, S. et al. (2007) Blood 109:4487.
    11. Kuckleburg, C.J. et al. (2012) J. Immunol. 188:2419.
    12. van Timmeren, M.M. and P. Heeringa (2012) Curr. Opin. Rheumatol. 24:8.
  • Long Name:
    Polycythemia Rubra Vera Protein 1
  • Entrez Gene IDs:
    57126 (Human); 68891 (Mouse)
  • Alternate Names:
    CD177 antigenNB1 GP; CD177 molecule; CD177; HNA-2a; HNA2Acell surface receptor; Human neutrophil alloantigen 2a; NB1 GP; NB1PRV-1; polycythemia rubra vera 1; Polycythemia rubra vera protein 1; PRV1; PRV1NB1 glycoprotein
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rabbit IgG Alexa Fluor® 700-conjugated Antibody

Ctrl IC1051N
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rabbit IgG Alexa Fluor® 700-conjugated Control

Ctrl IC105N
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse CD177 Alexa Fluor® 700-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee


Customer Information