Mouse CD229/SLAMF3 PE-conjugated Antibody

Discontinued Product

FAB2555P has been discontinued.
Detection of CD229/SLAMF3 in Mouse Splenocytes by Flow Cytometry.
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Mouse CD229/SLAMF3 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD229/SLAMF3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CD229 is observed.
Source
Monoclonal Rat IgG2A Clone # 312830
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD229/SLAMF3
Lys48-Phe454
Accession # AAH95921
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD229/SLAMF3 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of CD229/SLAMF3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD229/SLAMF3 PE‑conjugated Monoclonal Antibody (Catalog # FAB2555P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD229/SLAMF3

CD229, also known as T lymphocyte surface antigen Ly-9 and SLAMF3, is a type I transmembrane protein belonging to the immunoglobulin superfamily. It is also a member of the CD150/SLAM receptor family and is expressed on T and B lymphocytes. Mouse CD229 contains 2 Ig-like C2-type domains and 2 Ig-like V-type domains in its extracellular region. Two mouse alleles that differ in 6 extracellular amino acid residues have been reported. Human and mouse CD229 share 60% amino acid sequence identity in their extracellular regions.

Long Name
SLAM Family Member 3
Entrez Gene IDs
4063 (Human); 17085 (Mouse)
Alternate Names
CD229 antigen; CD229; cell-surface molecule Ly-9; hly9; Ly9; lymphocyte antigen 9Cell surface molecule Ly-9; mLY9; SLAMF3; T-lymphocyte surface antigen Ly-9

Product Datasheets

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