Mouse CD31/PECAM-1 Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse CD31/PECAM-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) DCC, rmICAM-1, -2, -5, rmMAdCAM-1, rmVCAM-1, recombinant porcine CD31/PECAM-1, recombinant human (rh) CD31/PECAM-1, rhCEACAM-1, rhSIGIRR, rhICAM‑3, or rhICAM-4 is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 693102
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with mouse CD31/PECAM-1
    Accession # Q08481
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD31/PECAM‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD31/PECAM‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB6874G, filled histogram) or isotype control antibody (Catalog # IC005G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD31/PECAM-1

PECAM-1 (platelet-endothelial cell adhesion molecule-1; also known as CD31) is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. CD31 is concentrated at cell-cell junctions and is required for transendothelial migration (TEM) (1‑3). The extracellular domain (ECD) of CD31 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains immunoregulatory tyrosine-based inhibitory and switch motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum CD31 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length CD31 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse CD31 ECD shows 77%, 63%, 63%, 63% and 61% amino acid (aa) identity with rat, human, canine, porcine and bovine CD31, respectively. CD31 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic CD31 adhesion in trans, combined with cycling of CD31 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, CD31 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. CD31-/- mice are deficient in chemokine-mediated chemotaxis (14).

  • References:
    1. Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
    2. Xie, Y. and Muller, W.A. (1993) Proc. Natl. Acad. Sci. USA 90:5569.
    3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
    4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
    5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
    6. Ilan, N. et al. (2001) FASEB J. 15:362.
    7. Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
    8. Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
    9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
    10. Mamdouh, Z. et al. (2003) Nature 421:748.
    11. Gao, C. et al. (2003) Blood 102:169.
    12. Falati, S. et al. (2006) Blood 107:535.
    13. Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
    14. Wu, Y. et al. (2005) J. Immunol. 175:3484.
  • Long Name:
    Platelet Endothelial Cell Adhesion Molecule 1
  • Entrez Gene IDs:
    5175 (Human); 18613 (Mouse); 29583 (Rat)
  • Alternate Names:
    adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; endoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC005G
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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