Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 310106
View all CD69 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD69
Gly64-Arg199
Accession # P37217

Specificity

Detects mouse CD69 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD69 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CD69 APC‑conjugated Antibody

Detection of CD69 antibody in CTLL‑2 Mouse Cell Line antibody by Flow Cytometry.

Detection of CD69 in CTLL‑2 Mouse Cell Line by Flow Cytometry.

CTLL-2 mouse cytotoxic T cell line was stained with Rat Anti-Mouse CD69 APC-conjugated Monoclonal Antibody (Catalog # FAB2386A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CD69 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CD69 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were (A) cultured with plate-bound anti-CD3 and anti-CD28 antibodies overnight, or (B) unstimulated, then stained with Rat Anti-Mouse CD69 APC-conjugated Monoclonal Antibody (Catalog # FAB2386A) and Rat anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P). Quadrant markers were set based on isotype control antibody (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD69 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: CTLL‑2 mouse cytotoxic T cell line, and Anti-CD3/CD28-activated mouse splenocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD69

CD69, also known as Leu 23, AIM, EA-1 and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells and platelets.

Alternate Names

CD69, EA-1, Leu23, MLR-3, p60

Entrez Gene IDs

969 (Human); 12515 (Mouse)

Gene Symbol

CD69

UniProt

P37217

Additional CD69 Products

Product Documents for Mouse CD69 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD69 APC‑conjugated Antibody

For research use only

Citations for Mouse CD69 APC‑conjugated Antibody

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FAQs for Mouse CD69 APC‑conjugated Antibody

Showing  1 - 2 of 2 FAQs Showing All

  • Q: What is the light chain isotype for Catalog #s FAB2386?

    A: The light chain isotype of Catalog #s FAB2386 is kappa.

  • Q: What is the light chain of the Mouse CD69 Antibody, Catalog #s MAB2386 and FAB2386, Clone # 1034124?

    A: Catalog #s MAB2386 and FAB2386 have a kappa light chain.

  • Q: What is the light chain isotype for Catalog #s FAB2386?

    A: The light chain isotype of Catalog #s FAB2386 is kappa.

  • Q: What is the light chain of the Mouse CD69 Antibody, Catalog #s MAB2386 and FAB2386, Clone # 1034124?

    A: Catalog #s MAB2386 and FAB2386 have a kappa light chain.

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies