Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Affinity Purification

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 310106
View all CD69 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD69
Gly64-Arg199
Accession # P37217

Specificity

Detects mouse CD69 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD69 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse CD69 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: CTLL‑2 mouse cytotoxic T cell line

Western Blot

1 µg/mL
Sample: Recombinant Mouse CD69 under non-reducing conditions only

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD69

CD69, also known as Leu 23, AIM, EA-1 and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells and platelets.

Alternate Names

CD69, EA-1, Leu23, MLR-3, p60

Entrez Gene IDs

969 (Human); 12515 (Mouse)

Gene Symbol

CD69

UniProt

P37217

Additional CD69 Products

Product Documents for Mouse CD69 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse CD69 Antibody

For research use only

Citations for Mouse CD69 Antibody

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FAQs for Mouse CD69 Antibody

Showing  1 - 2 of 2 FAQs Showing All

  • Q: What is the light chain isotype for Catalog # MAB2386?

    A: The light chain isotype of  Catalog # MAB2386 is kappa.

  • Q: What is the light chain of the Mouse CD69 Antibody, Catalog #s MAB2386 and FAB2386, Clone # 1034124?

    A: Catalog #s MAB2386 and FAB2386 have a kappa light chain.

  • Q: What is the light chain isotype for Catalog # MAB2386?

    A: The light chain isotype of  Catalog # MAB2386 is kappa.

  • Q: What is the light chain of the Mouse CD69 Antibody, Catalog #s MAB2386 and FAB2386, Clone # 1034124?

    A: Catalog #s MAB2386 and FAB2386 have a kappa light chain.

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies