Mouse CD69 Antibody

Catalog # Availability Size / Price Qty
MAB2386
MAB2386-SP

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Product Details
Citations (1)
FAQs
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Mouse CD69 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD69 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD69 is observed.
Source
Monoclonal Rat IgG2B Clone # 310106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD69
Gly64-Arg199
Accession # P37217
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CD69 under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
CTLL‑2 mouse cytotoxic T cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD69

CD69, also known as Leu 23, AIM, EA-1 and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells and platelets.

Entrez Gene IDs
969 (Human); 12515 (Mouse)
Alternate Names
activation inducer molecule (AIM/CD69); Activation inducer molecule; AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen); CD69 antigen; CD69 molecule; CD69; CLEC2CC-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; EA-1; early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leu23; Leukocyte surface antigen Leu-23; MLR-3; p60

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Citation for Mouse CD69 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Rai acts as a negative regulator of autoimmunity by inhibiting antigen receptor signaling and lymphocyte activation.
    Authors: Savino MT, Ortensi B, Ferro M, Ulivieri C, Fanigliulo D, Paccagnini E, Lazzi S, Osti D, Pelicci G, Baldari CT
    J. Immunol., 2009;182(1):301-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Affinity Purification

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