Mouse CD69 PE-conjugated Antibody Summary
Accession # P37217
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD69 in CTLL‑2 Mouse Cell Line by Flow Cytometry. CTLL-2 mouse cytotoxic T cell line was stained with Rat Anti-Mouse CD69 PE-conjugated Monoclonal Antibody (Catalog # FAB2386P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CD69 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were (A) cultured with plate-bound anti-CD3 and anti-CD28 antibodies overnight, or (B) unstimulated, then stained with Rat Anti-Mouse CD69 PE-conjugated Monoclonal Antibody (Catalog # FAB2386P) and Rat anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A). Quadrant markers were set based on isotype control antibody (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
CD69, also known as Leu 23, AIM, EA-1 and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells and platelets.
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