Mouse CD4 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB554P-100
FAB554P-025
Detection of CD4 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (3)
FAQs
Reviews (1)

Mouse CD4 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Reacts with mouse CD4, an antigen co-receptor on the T cell surface which interacts with MHC II molecules on antigen presenting cells.
Source
Monoclonal Rat IgG2B Clone # GK1.5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse CTL clone V4
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD4 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of CD4 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) or (B) Rat IgG2B Phycoerythrin Isotype Control (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD4

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

Entrez Gene IDs
920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 493775 (Feline)
Alternate Names
CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Product Datasheets

Citations for Mouse CD4 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Steady state migratory RelB+ langerin+ dermal dendritic cells mediate peripheral induction of antigen-specific CD4+ CD25+ Foxp3+ regulatory T cells.
    Authors: Azukizawa H, Dohler A, Kanazawa N, Nayak A, Lipp M, Malissen B, Autenrieth I, Katayama I, Riemann M, Weih F, Berberich-Siebelt F, Lutz MB
    Eur. J. Immunol., 2011;41(5):1420-34.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  2. Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells.
    Authors: Notley CA, Brown MA, Wright GP, Ehrenstein MR
    J. Immunol., 2011;186(8):4967-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  3. Differential IL-23 requirement for IL-22 and IL-17A production during innate immunity against Salmonella enterica serovar Enteritidis.
    Authors: Siegemund S, Schutze N, Schulz S, Wolk K, Nasilowska K, Straubinger RK, Sabat R, Alber G
    Int. Immunol., 2009;21(5):555-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow

FAQs

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Reviews for Mouse CD4 PE-conjugated Antibody

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Mouse CD4 PE-conjugated Antibody
By Anonymous on 01/25/2018
Application: Flow Sample Tested: Adult small intestine Species: Mouse