Mouse CD96 Antibody

R&D Systems | Catalog # AF5690

R&D Systems
Discontinued Product
AF5690 has been discontinued. View all CD96 products.

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CD96 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD96
Glu25-Met536
Accession # AAH52865

Specificity

Detects mouse CD96 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CD96 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse CD96 Antibody

Detection of Mouse CD96 antibody by Western Blot.

Detection of Mouse CD96 by Western Blot.

Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse CD96 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5690) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD96 at approximately 170 - 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse CD96 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: CTLL‑2 mouse cytotoxic T cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD96

Mouse CD96 (also Tactile) is a 170‑180 kDa member of the Ig-Superfamily. It is expressed on CD4+ and CD8+ T cells, NK and NKT cells, resting monocytes and gamma delta T cells. Mouse CD96 binds to CD155 and nectin-1, and likely participates in cell-to-cell adhesion. Mature mouse CD96 is a 581 amino acid (aa), type I transmembrane glycoprotein. It contains a 515 aa extracellular region (aa 22‑536) that contains three Ig-like domains, plus a 45 aa cytoplasmic region. The two N-terminal domains are V‑type (aa 24‑244), while the distal domain is a C-type structure (aa 250‑355). Unlike human, there is no splice variant in the second V‑type domain. There is, however, a potential isoform that shows a single Cys substitution for aa 437‑602. Over aa 1‑536, mouse CD96 shares 55% and 79% aa identity with human and rat CD96, respectively.

Alternate Names

CD96, TACTILE

Entrez Gene IDs

10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)

Gene Symbol

CD96

UniProt

AAH52865

Additional CD96 Products

Product Documents for Mouse CD96 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse CD96 Antibody

For research use only

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