Detection of Mouse CD96 by Western Blot.
Western blot shows lysates of CTLL‑2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse CD96 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5690) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD96 at approximately 170 - 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse CD96 (also Tactile) is a 170‑180 kDa member of the Ig-Superfamily. It is expressed on CD4+ and CD8+ T cells, NK and NKT cells, resting monocytes and gamma δ T cells. Mouse CD96 binds to CD155 and nectin-1, and likely participates in cell-to-cell adhesion. Mature mouse CD96 is a 581 amino acid (aa), type I transmembrane glycoprotein. It contains a 515 aa extracellular region (aa 22‑536) that contains three Ig-like domains, plus a 45 aa cytoplasmic region. The two N-terminal domains are V‑type (aa 24‑244), while the distal domain is a C-type structure (aa 250‑355). Unlike human, there is no splice variant in the second V‑type domain. There is, however, a potential isoform that shows a single Cys substitution for aa 437‑602. Over aa 1‑536, mouse CD96 shares 55% and 79% aa identity with human and rat CD96, respectively.
Entrez Gene IDs:
10225 (Human); 84544 (Mouse); 498079 (Rat)
CD96 antigenMGC22596; CD96 molecule; CD96; DKFZp667E2122; T cell-activated increased late expression protein; TACTILE; TACTILEincreased late expression; T-cell surface protein tactile
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