Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Polyclonal Goat IgG
Loading...

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD99-L2
Val20-Ala141
Accession # NP_612182

Specificity

Detects mouse CD99-L2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human CD99‑L2 and recombinant mouse CD99 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse CD99‑L2 Alexa Fluor® 405‑conjugated Antibody

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CD99-L2

CD99 antigen-like 2 (CD99-L2) is a 45 kDa type I transmembrane glycoprotein in the CD99 family of molecules (1-3). Mouse CD99-L2 cDNA encodes a 214 amino acid (aa) precursor with a 23 aa predicted signal sequence, a 116 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 54 aa cytoplasmic region (4). The ECD contains no N-linked glycosylation sites, but O-linked glycosylation is likely (1, 2). A long isoform with a 23 aa insert after aa 43 within the ECD is expressed in human, and probably in mouse (2, 5). Both long and short isoforms may have minor variants missing portions of the N-terminus of the mature protein (6). The ECD of mouse CD99-L2 short isoform shares 85%, 72% and 66% aa identity with rat, human, and bovine CD99-L2, respectively (3, 5). The mouse CD99 and CD99-L2 ECDs share only 31% aa identity, but both contain three conserved acidic motifs and are thought to originate from the same ancestral gene (1, 2). The nearly ubiquitous expression of CD99-L2 is similar to that of CD99. Mouse CD99-L2 shows highest in situ hybridization signals in neurons, cortical thymocytes, ganglia, ovarian granulosa cells, testis, and kidney, and detectable protein levels in lung, thymocytes, mouse leukocytes and vascular endothelial cells (2, 7). CD99‑L2 expression on endothelial cells is reported to mediate cell aggregation and neutrophil or monocyte extravasation to inflamed tissue in vivo, while CD99 mediates lymphocyte extravasation as well (3, 7).

Long Name

CD99 Molecule-like 2

Alternate Names

CD99B, CD99L2, MIC2L1

Entrez Gene IDs

83692 (Human); 171486 (Mouse); 171485 (Rat)

Gene Symbol

CD99L2

Additional CD99-L2 Products

Product Documents for Mouse CD99‑L2 Alexa Fluor® 405‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CD99‑L2 Alexa Fluor® 405‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Customer Reviews for Mouse CD99‑L2 Alexa Fluor® 405‑conjugated Antibody

There are currently no reviews for this product. Be the first to review Mouse CD99‑L2 Alexa Fluor® 405‑conjugated Antibody and earn rewards!

Have you used Mouse CD99‑L2 Alexa Fluor® 405‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies
Loading...