Mouse CD99-L2 Antibody

Catalog # Availability Size / Price Qty
AF5024
AF5024-SP
Detection of CD99‑L2 in bEnd.3 Mouse Cell Line by Flow Cytometry.
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Citations (1)
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Mouse CD99-L2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD99-L2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD99-L2 is observed and less than 5% cross-reactivity with recombinant mouse CD99 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD99-L2
Val20-Ala141
Accession # NP_612182
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CD99-L2 Fc Chimera (Catalog # 5024-CD)
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD99‑L2 in bEnd.3 Mouse Cell Line by Flow Cytometry. View Larger

Detection of CD99‑L2 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse CD99‑L2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5024, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD99-L2

CD99 antigen-like 2 (CD99-L2) is a 45 kDa type I transmembrane glycoprotein in the CD99 family of molecules (1 - 3). Mouse CD99-L2 cDNA encodes a 214 amino acid (aa) precursor with a 23 aa predicted signal sequence, a 116 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 54 aa cytoplasmic region (4). The ECD contains no N-linked glycosylation sites, but O-linked glycosylation is likely (1, 2). A long isoform with a 23 aa insert after aa 43 within the ECD is expressed in human, and probably in mouse (2, 5). Both long and short isoforms may have minor variants missing portions of the N-terminus of the mature protein (6). The ECD of mouse CD99-L2 short isoform shares 85%, 72% and 66% aa identity with rat, human, and bovine CD99-L2, respectively (3, 5). The mouse CD99 and CD99-L2 ECDs share only 31% aa identity, but both contain three conserved acidic motifs and are thought to originate from the same ancestral gene (1, 2). The nearly ubiquitous expression of CD99-L2 is similar to that of CD99. Mouse CD99-L2 shows highest in situ hybridization signals in neurons, cortical thymocytes, ganglia, ovarian granulosa cells, testis, and kidney, and detectable protein levels in lung, thymocytes, mouse leukocytes and vascular endothelial cells (2, 7). CD99‑L2 expression on endothelial cells is reported to mediate cell aggregation and neutrophil or monocyte extravasation to inflamed tissue in vivo, while CD99 mediates lymphocyte extravasation as well (3, 7).

References
  1. Park, S.H. et al. (2005) Gene 353:177.
  2. Suh, Y.H. et al. (2003) Gene 307:63.
  3. Schenkel, A.R. et al. (2007) Cell Commun. Adhes. 14:227.
  4. Entrez Accession # AAH31736.
  5. Entrez Accession # EDL26579.
  6. Entrez Accession # EDL26580 and CAM25451.
  7. Bixel, G. et al. (2007) Blood 109:5327.
Long Name
CD99 Molecule-like 2
Entrez Gene IDs
83692 (Human); 171486 (Mouse); 171485 (Rat)
Alternate Names
CD99 antigen; CD99 antigen-like 2; CD99 antigen-like protein 2; CD99 molecule-like 2; CD99B; CD99L2; CD99-L2; DKFZp761H2024; MIC2 like 1; MIC2L1; MIC2-like protein 1

Product Datasheets

Citation for Mouse CD99-L2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Interaction of CD99 with its paralog CD99L2 positively regulates CD99L2 trafficking to cell surfaces.
    Authors: Nam, Giri, Lee, Young-Kw, Lee, Hye Yeon, Ma, Min Jung, Araki, Masatake, Araki, Kimi, Lee, Seungbok, Lee, Im-Soon, Choi, Eun Youn
    J Immunol, 2013;191(11):5730-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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