Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD99
Gly25-Leu138
Accession # Q8VCN6

Specificity

Detects mouse CD99 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) PILR‑ alpha and rmPILR‑ beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CD99 PE‑conjugated Antibody

Detection of CD99 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CD99 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Goat Anti-Mouse CD99 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3905P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD99 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD99

CD99, also known as PILR-L and MIC2, is a 125 amino acid (aa), 24‑30 kDa glycosylated transmembrane glycoprotein expressed as a homodimer on the surface of endothelial, T and B cells, monocytes and stem cells. CD99 has been implicated in processes like homotypic cell adhesion, diapedesis and oncosuppression. The extracellular domain of the mouse CD99 protein used as immunogen has 60% aa sequence identity to rat CD99 and 43% aa sequence identity to the human CD99.

Alternate Names

CD99, MIC2, pilr-1, PILR-L

Entrez Gene IDs

4267 (Human); 673094 (Mouse); 652929 (Rat)

Gene Symbol

CD99

UniProt

Additional CD99 Products

Product Documents for Mouse CD99 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CD99 PE‑conjugated Antibody

For research use only

Citations for Mouse CD99 PE‑conjugated Antibody

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