Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CD99 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD99
Gly25-Leu138
Accession # Q8VCN6

Specificity

Detects mouse CD99 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CD99 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CD99 Antibody

Detection of CD99 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CD99 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Goat Anti-Mouse CD99 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3905, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (Catalog #NL002).

Applications for Mouse CD99 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample:
Mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CD99

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD99

CD99 (also known as PILR-L and MIC2) is a 125 amino acid, 24‑30 kDa glycosylated transmembrane glycoprotein expressed as a homodimer on the surface of endothelial, T and B cells, monocytes and stem cells. CD99 has been implicated in processes like homotypic cell adhesion, diapedesis and oncosupression. The extracellular domain of the mouse CD99 protein used as immunogen is 45% identical to the human counterpart.

Alternate Names

CD99, MIC2, pilr-1, PILR-L

Entrez Gene IDs

4267 (Human); 673094 (Mouse); 652929 (Rat)

Gene Symbol

CD99

UniProt

Q8VCN6

Additional CD99 Products

Product Documents for Mouse CD99 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse CD99 Antibody

For research use only

Citations for Mouse CD99 Antibody

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