CDO in Mouse Embryo.
CDO was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse CDO Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2429) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing central nervous system, cartilage and muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse CDO is a 190 kDa member of the Ig/FN-type III repeat subfamily of transmembrane (TM) proteins. The extracellular region contains five Ig-like domains and three FN-III modules. The molecule forms cis-complexes with itself and the related molecule termed BOC. Mouse CDO extracellular domain shares 95% and 85% aa identity with the corresponding regions of rat and human CDO, respectively.
Cell Adhesion Molecule-related/Down-regulated by Oncogenes
Entrez Gene IDs:
50937 (Human); 57810 (Mouse)
CDO; Cdon homolog (mouse); CDON; CDOORCAM; cell adhesion molecule-related/down-regulated by oncogenes; MGC111524; ORCAM; surface glycoprotein, Ig superfamily member
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