Mouse Cerberus 1 Antibody

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Product Details
Citations (3)
Supplemental Products
Reviews (1)

Mouse Cerberus 1 Antibody Summary

Species Reactivity
Detects mouse Cerberus 1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human Cerberus, recombinant mouse (rm) DAN, or rmGremlin.
Monoclonal Rat IgG2A Clone # 225807
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse Cerberus 1
Accession # O55233
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Mouse Cerberus 1 (Catalog # 1986-CB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cerberus 1

Cerberus 1, also called DAND4, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and COCO/Dante (DAND5). DAN family members contain a cysteine-knot domain that is homologous to that found in other TGF-beta superfamily ligands (1, 2). Mature mouse Cerberus 1 shares 40%, 29%, and 66% amino acid (aa) sequence identity with chick, Xenopus, and human Cerberus 1, respectively. Within the cysteine-knot domain, it shares 24-37% aa sequence identity with mouse DAN, Gremlin, PRDC, and COCO. Cerberus 1 is a secreted 38 kDa glycoprotein that forms disulfide-linked homodimers (3). Cerberus-S, which is generated by proteolysis in Xenopus, is a short version of the molecule and includes the C-terminal cysteine-knot domain (4). At the onset of gastrulation, Cerberus 1 is transiently expressed in anterior endodermal structures in response to Nodal and Shh (3, 5-9). Cerberus 1 binds BMP-4 and Nodal and inhibits their activities. Xenopus Cerberus has also been shown to bind Xenopus Wnt8. These inhibitory functions of Cerberus favor mesodermal development in the anterior region of the gastrula and suppresses posterior mesodermal differentiation (3, 4, 6, 8, 10-12). In chick and Xenopus, Cerberus 1 also regulates, but is not required for embryonic left-right polarization, neurulation, and head and heart induction (4-8, 13).

  1. Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1.
  2. Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
  3. Biben, C. et al. (1998) Dev. Biol. 194:135.
  4. Piccolo, S. et al. (1999) Nature 397:707.
  5. Zhu, L. et al. (1999) Curr. Biol. 9:931.
  6. Tavares, A.T. et al. (2007) Development 134:2051.
  7. Foley, A.C. et al. (2007) Dev. Biol. 303:57.
  8. Bouwmeester, T. et al. (1996) Nature 382:595.
  9. Bertocchini, F. and C.D. Stern (2002) Dev. Cell 3:735.
  10. Glinka, A. et al. (1997) Nature 389:517.
  11. Perea-Gomez, A. et al. (2002) Dev. Cell 3:745.
  12. Takaoka, K. et al. (2007) Curr. Opin. Genet. Dev. 17:344
  13. Simpson, E.H. et al. (1999) Dev. Biol. 213:202.
Entrez Gene IDs
9350 (Human); 12622 (Mouse)
Alternate Names
CER1; cerberus 1 (Xenopus laevis) homolog (cysteine knot superfamily); Cerberus 1; cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis); cerberus; cerberus-related 1; Cerberus-related protein; DAN domain family member 4; DAND4; MGC119894; MGC119895; MGC96951

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Citations for Mouse Cerberus 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Basement membrane remodelling regulates mouse embryogenesis
    Authors: C Kyprianou, N Christodou, RS Hamilton, W Nahaboo, DS Boomgaard, G Amadei, I Migeotte, M Zernicka-G
    Nature, 2020;582(7811):253-258.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling in embryos by shedding CRIPTO
    Authors: Taroh Kinoshita
    J. Cell Biol., 2016;215(5):705-718.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. The anterior visceral endoderm of the mouse embryo is established from both preimplantation precursor cells and by de novo gene expression after implantation.
    Authors: Torres-Padilla ME, Richardson L, Kolasinska P, Meilhac SM, Luetke-Eversloh MV, Zernicka-Goetz M
    Dev. Biol., 2007;309(1):97-112.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC


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Mouse Cerberus 1 Antibody
By Anonymous on 10/26/2015
Application: ICC/IF Sample Tested: E6.5 mouse embryo fixed in 4% PFA Species: Mouse

E6.5 mouse embryo fixed in 4% PFA was stained with this antibody. DAPI was used for nuclear counterstain. Cerberus1 positive cells were seen in the anterior visceral endoderm.
Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: PBS-T
Dilution: 1:100