Mouse Cerberus 1 Antibody
Mouse Cerberus 1 Antibody Summary
Asp18-Pro272
Accession # O55233
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cerberus 1
Cerberus 1, also called DAND4, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and COCO/Dante (DAND5). DAN family members contain a cysteine-knot domain that is homologous to that found in other TGF-beta superfamily ligands (1, 2). Mature mouse Cerberus 1 shares 40%, 29%, and 66% amino acid (aa) sequence identity with chick, Xenopus, and human Cerberus 1, respectively. Within the cysteine-knot domain, it shares 24-37% aa sequence identity with mouse DAN, Gremlin, PRDC, and COCO. Cerberus 1 is a secreted 38 kDa glycoprotein that forms disulfide-linked homodimers (3). Cerberus-S, which is generated by proteolysis in Xenopus, is a short version of the molecule and includes the C-terminal cysteine-knot domain (4). At the onset of gastrulation, Cerberus 1 is transiently expressed in anterior endodermal structures in response to Nodal and Shh (3, 5-9). Cerberus 1 binds BMP-4 and Nodal and inhibits their activities. Xenopus Cerberus has also been shown to bind Xenopus Wnt8. These inhibitory functions of Cerberus favor mesodermal development in the anterior region of the gastrula and suppresses posterior mesodermal differentiation (3, 4, 6, 8, 10-12). In chick and Xenopus, Cerberus 1 also regulates, but is not required for embryonic left-right polarization, neurulation, and head and heart induction (4-8, 13).
- Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1.
- Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
- Biben, C. et al. (1998) Dev. Biol. 194:135.
- Piccolo, S. et al. (1999) Nature 397:707.
- Zhu, L. et al. (1999) Curr. Biol. 9:931.
- Tavares, A.T. et al. (2007) Development 134:2051.
- Foley, A.C. et al. (2007) Dev. Biol. 303:57.
- Bouwmeester, T. et al. (1996) Nature 382:595.
- Bertocchini, F. and C.D. Stern (2002) Dev. Cell 3:735.
- Glinka, A. et al. (1997) Nature 389:517.
- Perea-Gomez, A. et al. (2002) Dev. Cell 3:745.
- Takaoka, K. et al. (2007) Curr. Opin. Genet. Dev. 17:344
- Simpson, E.H. et al. (1999) Dev. Biol. 213:202.
Product Datasheets
Citations for Mouse Cerberus 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Basement membrane remodelling regulates mouse embryogenesis
Authors: C Kyprianou, N Christodou, RS Hamilton, W Nahaboo, DS Boomgaard, G Amadei, I Migeotte, M Zernicka-G
Nature, 2020;582(7811):253-258.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling in embryos by shedding CRIPTO
Authors: Taroh Kinoshita
J. Cell Biol., 2016;215(5):705-718.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The anterior visceral endoderm of the mouse embryo is established from both preimplantation precursor cells and by de novo gene expression after implantation.
Authors: Torres-Padilla ME, Richardson L, Kolasinska P, Meilhac SM, Luetke-Eversloh MV, Zernicka-Goetz M
Dev. Biol., 2007;309(1):97-112.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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E6.5 mouse embryo fixed in 4% PFA was stained with this antibody. DAPI was used for nuclear counterstain. Cerberus1 positive cells were seen in the anterior visceral endoderm.
Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: PBS-T
Dilution: 1:100
