Mouse Complement Component C5a R1 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    This antibody detects mouse Complement Component C5a R1 in direct ELISAs.
  • Source
    Monoclonal Rat IgG2B Clone # 583837
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with mouse Complement Component C5a R1
    Accession # P30993
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Complement Component C5a R1 in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse Complement Component C5a R1 APC‑conjugated Monoclonal Antibody (Catalog # FAB6467A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Complement Component C5a R1
Complement Component C5a R1, also known as C5a ligand and CD88, is a 7TM protein expressed on myeloid, endothelial, epithelial, and smooth muscle cells. C5a R1 binds the activated complement anaphylatoxin C5a. In established allergic environments, this triggers neutrophil and eosinophil chemotaxis and the release of proinflammatory mediators. In contrast, C5a R1/C5a interactions are protective during allergen sensitization. Mouse C5a R1 shares 66% and 77% amino acid sequence identity with human and rat C5a R, respectively.
  • Long Name:
    Complement Component C5a Receptor 1
  • Entrez Gene IDs:
    728 (Human); 12273 (Mouse)
  • Alternate Names:
    C5a anaphylatoxin chemotactic receptor; C5A; C5aR; C5a-R; C5AR1; C5ARC5a anaphylatoxin receptor; C5R1C5a ligand; CD88 antigen; CD88; complement component 5 receptor 1 (C5a ligand); complement component 5 receptor 1; complement component 5a receptor 1; Complement Component C5aR1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B APC‑conjugated Isotype Control

Ctrl IC013A 3  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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