Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 583837
View all Complement Component C5aR1 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with mouse Complement Component C5a R1
Accession # P30993

Specificity

This antibody detects mouse Complement Component C5a R1 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Complement Component C5aR1 APC-conjugated Antibody

Detection of Complement Component C5a R1 antibody in J774A.1 Mouse Cell Line antibody by Flow Cytometry.

Detection of Complement Component C5a R1 in J774A.1 Mouse Cell Line by Flow Cytometry.

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse Complement Component C5a R1 APC-conjugated Monoclonal Antibody (Catalog # FAB6467A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Complement Component C5aR1 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Complement Component C5aR1

Complement Component C5a R1, also known as C5a ligand and CD88, is a 7TM protein expressed on myeloid, endothelial, epithelial, and smooth muscle cells. C5a R1 binds the activated complement anaphylatoxin C5a. In established allergic environments, this triggers neutrophil and eosinophil chemotaxis and the release of proinflammatory mediators. In contrast, C5a R1/C5a interactions are protective during allergen sensitization. Mouse C5a R1 shares 66% and 77% amino acid sequence identity with human and rat C5a R, respectively.

Long Name

Complement Component C5a Receptor 1

Alternate Names

C5AR1, CD88, Complement Component C5a R1

Entrez Gene IDs

728 (Human); 12273 (Mouse)

Gene Symbol

C5AR1

UniProt

P30993

Additional Complement Component C5aR1 Products

Product Documents for Mouse Complement Component C5aR1 APC-conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Complement Component C5aR1 APC-conjugated Antibody

For research use only

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