Mouse Complement Component C5aR1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6467A
Detection of Complement Component C5a R1 in J774A.1 Mouse Cell Line by Flow Cytometry.
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Mouse Complement Component C5aR1 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
This antibody detects mouse Complement Component C5a R1 in direct ELISAs.
Source
Monoclonal Rat IgG2B Clone # 583837
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with mouse Complement Component C5a R1
Accession # P30993
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Complement Component C5a R1 antibody in J774A.1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Complement Component C5a R1 in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse Complement Component C5a R1 APC-conjugated Monoclonal Antibody (Catalog # FAB6467A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Complement Component C5aR1

Complement Component C5a R1, also known as C5a ligand and CD88, is a 7TM protein expressed on myeloid, endothelial, epithelial, and smooth muscle cells. C5a R1 binds the activated complement anaphylatoxin C5a. In established allergic environments, this triggers neutrophil and eosinophil chemotaxis and the release of proinflammatory mediators. In contrast, C5a R1/C5a interactions are protective during allergen sensitization. Mouse C5a R1 shares 66% and 77% amino acid sequence identity with human and rat C5a R, respectively.

Long Name
Complement Component C5a Receptor 1
Entrez Gene IDs
728 (Human); 12273 (Mouse)
Alternate Names
C5a anaphylatoxin chemotactic receptor; C5A; C5aR; C5a-R; C5AR1; C5ARC5a anaphylatoxin receptor; C5R1C5a ligand; CD88 antigen; CD88; complement component 5 receptor 1 (C5a ligand); complement component 5 receptor 1; complement component 5a receptor 1; Complement Component C5a R1; Complement Component C5aR1

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