Mouse Complement Component C5aR1 APC-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry View Larger

Detection of Complement Component C5a R1 in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse Complement Component C5a R1 APC-conjugated Monoclonal Antibody (Catalog # FAB6467A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Background: Complement Component C5aR1

Complement Component C5a R1, also known as C5a ligand and CD88, is a 7TM protein expressed on myeloid, endothelial, epithelial, and smooth muscle cells. C5a R1 binds the activated complement anaphylatoxin C5a. In established allergic environments, this triggers neutrophil and eosinophil chemotaxis and the release of proinflammatory mediators. In contrast, C5a R1/C5a interactions are protective during allergen sensitization. Mouse C5a R1 shares 66% and 77% amino acid sequence identity with human and rat C5a R, respectively.

Product Datasheets

Related Research Areas

• Complement Pathway
• G Protein-Coupled Receptors in Immune Responses
• Myeloid-derived Suppressor Cells (MDSC)
• Serine Proteases and Regulators