Mouse DEC-205/CD205 Antibody (561118) [APC]

R&D Systems | Catalog # FAB5975A

R&D Systems
Discontinued Product
FAB5975A has been discontinued. View all DEC-205/CD205 products.

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 561118
View all DEC-205/CD205 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse DEC-205/CD205
Cys216-Pro503
Accession # Q60767

Specificity

Detects mouse DEC-205/CD205 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse DEC-205/CD205 Antibody (561118) [APC]

Detection of DEC-205/CD205 antibody in Mouse Lymph Node-derived CD11c+CD8lowCells antibody by Flow Cytometry.

Detection of DEC‑205/CD205 in Mouse Lymph Node-derived CD11c+CD8lowCells by Flow Cytometry.

Mouse lymph node-derived CD11c+CD8lowcells were stained with Rat Anti-Mouse DEC-205/CD205 APC-conjugated Monoclonal Antibody (Catalog # FAB5975A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse DEC-205/CD205 Antibody (561118) [APC]

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse lymph node-derived CD11c+ CD8low cells

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: DEC-205/CD205

DEC-205 (Dendritic and thymic Epithelial Cell-205; also Ly-75 and CD205) is a 200-205 kDa member of the mannose receptor family of molecules. In mouse, it is widely expressed, being found on B cells, Langerhans cells, bone marrow-derived dendritic cells, neutrophils, plus respiratory, intestinal, and thymic cortical epithelium. DEC-205 serves as a recognition/endocytic receptor for dying cells, and likely participates in the induction of self-tolerance. Mature mouse DEC-205 is a 1696 amino acid (aa) type I transmembrane glycoprotein (aa 28-1723). It contains a 1640 aa extracellular domain (ECD) (aa 28-1667) and a 31 aa cytoplasmic tail. The ECD is highly modular, containing a ricin B-type lectin domain (aa 33-182), a FN type II domain (aa 164-211) and nine sequential C-type lectin domains (aa 225-1662). There are at least two potential isoform variants. One shows a two aa substitution for aa 866-1723, and another shows a 64 aa substitution for aa 734-1723. Over aa 216-503, mouse DEC-205 shares 87% and 79% aa identity with rat and human DEC-205, respectively.

Alternate Names

CD205, DEC205, Ly75

Entrez Gene IDs

4065 (Human); 17076 (Mouse)

Gene Symbol

LY75

UniProt

Q60767

Additional DEC-205/CD205 Products

Product Documents for Mouse DEC-205/CD205 Antibody (561118) [APC]

Certificate of Analysis

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Product Specific Notices for Mouse DEC-205/CD205 Antibody (561118) [APC]

For research use only

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FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways
Dendritic Cell Lineage Development Pathway Thumbnail