Mouse Dectin-2/CLEC6A alpha Isoform APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse Dectin‑2 alpha /CLEC6A alpha Isoform in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human DLEC is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Dectin‑2 alpha /CLEC6A
    Accession # Q9JKF4
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Dectin‑2/CLEC6A in Mouse Dendritic Cells by Flow Cytometry. Mouse bone marrow-derived dendritic cells were stained with Goat Anti-Mouse
Dectin‑2/CLEC6A alpha Isoform APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1525A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Dectin-2/CLEC6A

Dectin-2 alpha, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2 alpha, CLEC4A/DCIR, CLEC4B/DCAR, CLEC4C/DLEC, CLEC4D/MCL, and CLEC4E/mincle constitute a subgroup of these molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD) and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature mouse Dectin-2 alpha consists of a short cytoplasmic tail, a transmembrane segment, and an ECD with a stalk region and one CRD (3, 4). Alternate splicing leads to partial deletion of the transmembrane segment and stalk ( beta isoform) or a portion of the CRD ( gamma isoform) (4). The full length Dectin-2 alpha isoform is a 27 kDa molecule that is primarily expressed on the surface of tissue macrophages and their precursors (3‑6). The CRD of Dectin-2 alpha contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 alpha selectively interacts with high mannose structures in the Man9GlcNAc2 configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit in macrophages (8). Ligation of Dectin-2 alpha induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 alpha is upregulated in vivo by inflammatory stimuli and UV-B irradiation (6). It mediates the breaking of UV-induced tolerance by interacting with CD4+CD25+ regulatory T cells which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (9). Within the ECD, mouse Dectin-2 alpha shares 71% aa sequence identity with human and bovine Dectin-2.

  • References:
    1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
    2. Kanazawa, N. et al. (2004) Immunobiology 209:179.
    3. Fernandes, M.J. et al. (1999) Cancer Res. 59:2709.
    4. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:11957.
    5. Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.
    6. Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.
    7. McGreal, E.P. et al. (2006) Glycobiology 16:422.
    8. Sato, K. et al. (2006) J. Biol. Chem. 281:38854.
    9. Aragane, Y. et al. (2003) J. Immunol. 171:3801.
  • Entrez Gene IDs:
    93978 (Human); 56620 (Mouse)
  • Alternate Names:
    CLEC4N; CLEC6A; CLECSF10; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10; C-type lectin domain family 6 member A; C-type lectin domain family 6, member A; C-type lectin superfamily member 10; DC-associated C-type lectin 2; dectin 2; Dectin2; dectin-2; Dendritic cell-associated C-type lectin 2
Related Research Areas
Isotype Controls
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Goat IgG APC-conjugated Antibody

Ctrl IC108A 2  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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