Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 355022
View all EBI3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL‑27 heterodimer
Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28)
Accession # O35228 (EBI3) & Q8K3I6 (p28)

Specificity

Detects mouse IL-27/IL-35 EBI3 Subunit in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human IL‑27/IL-35 EBI3 subunit.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse EBI3 APC-conjugated Antibody

Detection of IL-27/IL-35 EBI3 Subunit antibody in Mouse splenocytes antibody by Flow Cytometry.

Detection of IL-27/IL-35 EBI3 Subunit in Mouse splenocytes by Flow Cytometry.

Mouse splenocytes untreated (open histogram) or treated with LPS (filled histogram) were stained with Rat Anti-Mouse IL-27/IL-35 EBI3 Subunit APC-conjugated Monoclonal Antibody (Catalog # IC18341A) or isotype control antibody (Catalog # IC006A, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Mouse EBI3 APC-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes treated with LPS, fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

Reviewed Applications

Read 2 reviews rated 4.5 using IC18341A in the following applications:

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: EBI3

IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide anda 209 aa mature protein with two fibronectin type III domains.

Long Name

Epstein-Barr virus induced gene 3

Alternate Names

EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3

Entrez Gene IDs

10148 (Human); 50498 (Mouse); 680609 (Rat); 102144621 (Cynomolgus Monkey)

Gene Symbol

EBI3

UniProt

O35228 (EBI3) & Q8K3I6 (p28)

Additional EBI3 Products

Product Documents for Mouse EBI3 APC-conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse EBI3 APC-conjugated Antibody

For research use only

Related Research Areas

Regulatory T Cells (Tregs)

Citations for Mouse EBI3 APC-conjugated Antibody

Customer Reviews for Mouse EBI3 APC-conjugated Antibody (2)

4.5 out of 5
2 Customer Ratings
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  • Mouse IL-27/IL-35 EBI3 Subunit APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Mouse splenocytes
    Species: Mouse
    Verified Customer | Posted 04/13/2017
    Mouse EBI3 APC-conjugated Antibody IC18341A
  • Mouse IL-27/IL-35 EBI3 Subunit APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Splenic T cells
    Species: Mouse
    Verified Customer | Posted 07/27/2016

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