Mouse EphA2 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB639G-100UG
Detection  of EphA2 in MS‑1 Mouse Cell Line by Flow Cytometry.
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Mouse EphA2 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse EphA2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human EphA1, recombinant mouse (rm) EphA3, 4, 6, 7, 8, recombinant rat EphA5, rmEphB1, 2, 3, 4, or 6 is observed.
Source
Monoclonal Rat IgG2B Clone # 233720
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphA2
Ala22-Ala535 (predicted)
Accession # AAA82113
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of EphA2 in MS‑1 Mouse Cell Line by Flow Cytometry. View Larger

Detection of EphA2 in MS‑1 Mouse Cell Line by Flow Cytometry. MS‑1 mouse pancreatic islet endothelial cell line was stained with Rat Anti-Mouse EphA2 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB639G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: EphA2

EphA2, also known as Eck, Myk2, and Sek2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA2 has been shown to bind ephrin-A3, ephrin-A1, ephrin-A5, ephrin-A4, and ephrin-A2 (2, 3). The extracellular domains of mouse and human EphA2 share greater than 92% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.
Entrez Gene IDs
1969 (Human); 13836 (Mouse)
Alternate Names
ARCC2; EC 2.7.10; EC 2.7.10.1; Eck; ECKepithelial cell receptor protein tyrosine kinase; EPH receptor A2; EphA2; ephrin type-A receptor 2; Epithelial cell kinase; Myk2; Sek2; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK

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