Mouse EphA2 Antibody

Catalog # Availability Size / Price Qty
MAB639
MAB639-SP
Mouse EphA2 Antibody in Data
2 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Mouse EphA2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse EphA2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human EphA1, recombinant mouse (rm) EphA3, 4, 6, 7, 8, recombinant rat EphA5, rmEphB1, 2, 3, 4, or 6 is observed.
Source
Monoclonal Rat IgG2B Clone # 233720
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphA2
Ala22-Ala535 (predicted)
Accession # AAA82113
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse EphA2 Fc Chimera (Catalog # 639-A2) under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of EphA2 in D3 Mouse Cell Line by Flow Cytometry. View Larger

Detection of EphA2 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse EphA2 Monoclonal Antibody (Catalog # MAB639, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of EphA2 in HEK293 Human Cell Line Transfected with Mouse EphA2 by Flow Cytometry. View Larger

Detection of EphA2 in HEK293 Human Cell Line Transfected with Mouse EphA2 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse EphA2 was stained with Rat Anti-Mouse EphA2 Monoclonal Antibody (Catalog # MAB639, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphA2

EphA2, also known as Eck, Myk2, and Sek2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA2 has been shown to bind ephrin-A3, ephrin-A1, ephrin-A5, ephrin-A4, and ephrin-A2 (2, 3). The extracellular domains of mouse and human EphA2 share greater than 92% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.
Entrez Gene IDs
1969 (Human); 13836 (Mouse)
Alternate Names
ARCC2; EC 2.7.10; EC 2.7.10.1; Eck; ECKepithelial cell receptor protein tyrosine kinase; EPH receptor A2; EphA2; ephrin type-A receptor 2; Epithelial cell kinase; Myk2; Sek2; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK

Product Datasheets

Citations for Mouse EphA2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Dysregulation of EGFR pathway in EphA2 cell subpopulation significantly associates with poor prognosis in colorectal cancer
    Clin Cancer Res, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. EphA2 receptor mediates increased vascular permeability in lung injury due to viral infection and hypoxia.
    Authors: Cercone MA, Schroeder W, Schomberg S, Carpenter TC
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(5):L856-63.
    Species: Rat
    Sample Types: In Vivo
    Applications: Neutralization
  3. Tissue transglutaminase clusters soluble A-type ephrins into functionally active high molecular weight oligomers.
    Authors: Alford SC, Bazowski J, Lorimer H, Elowe S, Howard PL
    Exp. Cell Res., 2007;313(20):4170-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse EphA2 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Mouse EphA2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse EphA2 Antibody
By Anonymous on 12/21/2018
Application: Flow Sample Tested: Brain (caudate nucleus) Species: Mouse