Mouse Fc gamma RIII (CD16) APC‑conjugated Antibody

R&D Systems | Catalog # FAB19601A

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 275003
View all Fc gamma RIII (CD16) Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma  RIII
Ala31-Thr215
Accession # Q5D5J5

Specificity

Detects mouse Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Fcrl3/CD16-2, rmFc gamma RIA or rhFc gamma RIIA is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Fc gamma RIII (CD16) APC‑conjugated Antibody

Detection of Fc gamma RIII (CD16) antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of Fc gamma RIII (CD16) in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Fc gamma RIII (CD16) APC-conjugated Monoclonal Antibody (Catalog # FAB19601A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Fc gamma RIII (CD16) APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Reviewed Applications

Read 2 reviews rated 3 using FAB19601A in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Fc gamma RIII (CD16)

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1-3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors
(~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5). Mouse CD16 is encoded by a single gene. The protein product is a type I transmembrane protein having two extracellular Ig-like domains. It is expressed on a variety of myeloid and lymphoid cells (4) and associates with Fc R gamma to deliver an activating signal upon ligand binding (5). Mouse CD32 is closely related to mouse CD16 throughout its extracellular domain (95% amino acid sequence identity), but has a divergent cytoplasmic domain and functions as an inhibitory receptor. Together these proteins constitute an activating/inhibiting receptor pair to regulate immune responses (5).

References

  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181. 
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.

Long Name

Fc gamma Receptor III

Alternate Names

FcgRIII

Entrez Gene IDs

14131 (Mouse); 102140945 (Cynomolgus Monkey)

Gene Symbol

FCGR3

UniProt

Q5D5J5

Additional Fc gamma RIII (CD16) Products

Product Documents for Mouse Fc gamma RIII (CD16) APC‑conjugated Antibody

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Product Specific Notices for Mouse Fc gamma RIII (CD16) APC‑conjugated Antibody

For research use only

Citations for Mouse Fc gamma RIII (CD16) APC‑conjugated Antibody

Customer Reviews for Mouse Fc gamma RIII (CD16) APC‑conjugated Antibody (2)

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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 23585480
    Species: Mouse
    Verified Customer | Posted 01/21/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 23585480
    Species: Mouse
    Verified Customer | Posted 01/21/2015

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