Mouse Fc gamma RIII (CD16) Antibody

Catalog # Availability Size / Price Qty
MAB19601
MAB19601-SP
Detection of Fc gamma RIII (CD16) in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (14)
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Mouse Fc gamma RIII (CD16) Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Fcrl3/CD16-2, rmFc gamma RIA or rhFc gamma RIIA is observed.
Source
Monoclonal Rat IgG2A Clone # 275003
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma  RIII
Ala31-Thr215
Accession # Q5D5J5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Fc gamma RIII (CD16) antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Fc gamma RIII (CD16) in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Fc gamma RIII (CD16) Monoclonal Antibody (Catalog # MAB19601, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIII (CD16)

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1‑3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10-8‑10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6‑10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5). Mouse CD16 is encoded by a single gene. The protein product is a type I transmembrane protein having two extracellular Ig-like domains. It is expressed on a variety of myeloid and lymphoid cells (4) and associates with Fc R gamma to deliver an activating signal upon ligand binding (5). Mouse CD32 is closely related to mouse CD16 throughout its extracellular domain (95% amino acid sequence identity), but has a divergent cytoplasmic domain and functions as an inhibitory receptor. Together these proteins constitute an activating/inhibiting receptor pair to regulate immune responses (5).

References
  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181. 
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.
Long Name
Fc gamma Receptor III
Entrez Gene IDs
14131 (Mouse); 102140945 (Cynomolgus Monkey)
Alternate Names
CD16; CD16A; Fc fragment of IgG receptor IIIa; Fc gamma RIII; FCG3; FCGR3; FcgRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20

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Citations for Mouse Fc gamma RIII (CD16) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. IL-4 controls activated neutrophil FcgammaR2b expression and migration into inflamed joints
    Authors: SK Panda, G Wigerblad, L Jiang, Y Jiménez-An, VS Iyer, Y Shen, SV Boddul, AO Guerreiro-, B Raposo, Z Kasza, F Wermeling
    Proc. Natl. Acad. Sci. U.S.A., 2020;117(6):3103-3113.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Laquinimod, a prototypic quinoline-3-carboxamide and aryl hydrocarbon receptor agonist, utilizes a CD155-mediated natural killer/dendritic cell interaction to suppress CNS autoimmunity
    Authors: M Ott, E Avendaño-G, E Ullrich, C Dreyer, J Strauss, M Harden, M Schön, MP Schön, G Bernhardt, C Stadelmann, C Wegner, W Brück, S Nessler
    J Neuroinflammation, 2019;16(1):49.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. A noncanonical role for the engulfment gene ELMO1 in neutrophils that promotes inflammatory arthritis
    Authors: S Arandjelov, JSA Perry, CD Lucas, KK Penberthy, TH Kim, M Zhou, DA Rosen, TY Chuang, AM Bettina, LS Shankman, AH Cohen, A Gaultier, TP Conrads, M Kim, MR Elliott, KS Ravichandr
    Nat. Immunol., 2019;20(2):141-151.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Ligand-Blocking and Membrane-Proximal Domain Targeting Anti-OX40 Antibodies Mediate Potent T Cell-Stimulatory and Anti-Tumor Activity
    Authors: P Zhang, GH Tu, J Wei, P Santiago, LR Larrabee, S Liao-Chan, T Mistry, ML Chu, T Sai, K Lindquist, H Long, J Chaparro-R, S Salek-Arda, YA Yeung
    Cell Rep, 2019;27(11):3117-3123.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. HoxB8 neutrophils replicate Fc? receptor and integrin-induced neutrophil signaling and functions
    Authors: JY Chu, B McCormick, G Mazelyte, M Michael, S Vermeren
    J. Leukoc. Biol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. DC subset-specific induction of T cell responses upon antigen uptake via Fc? receptors in vivo
    Authors: CH Lehmann, A Baranska, GF Heidkamp, L Heger, K Neubert, JJ Lühr, A Hoffmann, KC Reimer, C Brückner, S Beck, M Seeling, M Kie beta ling, D Soulat, AB Krug, JV Ravetch, JH Leusen, F Nimmerjahn, D Dudziak
    J. Exp. Med., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  7. C5aR activation in the absence of C5a - a new disease mechanism of Autoimmune Hemolytic Anemia in mice
    Authors: SN Syed, E Rau, M Ziegelmann, G Sogkas, B Brüne, RE Schmidt
    Eur. J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. IgG subclasses determine pathways of anaphylaxis in mice
    Authors: H Beutier, CM Gillis, B Iannascoli, O Godon, P England, R Sibilano, LL Reber, SJ Galli, MS Cragg, N Van Rooije, DA Mancardi, P Bruhns, F Jönsson
    J. Allergy Clin. Immunol., 2017;139(1):269-280.e7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Rapid desensitization of mice with anti-FcgammaRIIb/FcgammaRIII mAb safely prevents IgG-mediated anaphylaxis.
    Authors: Khodoun M, Kucuk Z, Strait R, Krishnamurthy D, Janek K, Clay C, Morris S, Finkelman F
    J Allergy Clin Immunol, 2013;132(6):1375-87.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Functional Assay
  10. Cutting edge: FcgammaRIII (CD16) and FcgammaRI (CD64) are responsible for anti-glycoprotein 75 monoclonal antibody TA99 therapy for experimental metastatic B16 melanoma.
    Authors: Albanesi M, Mancardi D, Macdonald L, Iannascoli B, Zitvogel L, Murphy A, Daeron M, Leusen J, Bruhns P
    J Immunol, 2012;189(12):5513-7.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  11. beta5 integrin is the major contributor to the alphaVintegrin-mediated blockade of HIV-1 replication.
    Authors: Ballana E, Pauls E, Clotet B, Perron-Sierra F, Tucker GC, Este JA
    J. Immunol., 2011;186(1):464-70.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  12. Mouse and human neutrophils induce anaphylaxis.
    Authors: Jonsson F, Mancardi DA, Kita Y, Karasuyama H, Iannascoli B, Van Rooijen N, Shimizu T, Daeron M, Bruhns P
    J. Clin. Invest., 2011;121(4):1484-96.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  13. Facilitated antigen uptake and timed exposure to TLR ligands dictate the antigen-presenting potential of plasmacytoid DCs.
    Authors: Kool M, Geurtsvankessel C, Muskens F, Madeira FB, van Nimwegen M, Kuipers H, Thielemans K, Hoogsteden HC, Hammad H, Lambrecht BN
    J. Leukoc. Biol., 2011;90(6):1177-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Both FcgammaRIV and FcgammaRIII are essential receptors mediating type II and type III autoimmune responses via FcRgamma-LAT-dependent generation of C5a.
    Authors: Syed SN, Konrad S, Wiege K, Nieswandt B, Nimmerjahn F, Schmidt RE, Gessner JE
    Eur. J. Immunol., 2009;39(12):3343-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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