Mouse FCRL1/FcRH1 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse FCRL1/FcRH1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human FCRL1/FcRH1 is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 745916
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse FCRL1/FcRH1
    Accession # Q8R4Y0
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of FCRL1/FcRH1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse FCRL1/FcRH1 PE‑conjugated Monoclonal Antibody (Catalog # FAB7286P) or (B) Rat IgG1 Phycoerythrin Isotype Control (Catalog # IC005P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: FCRL1/FcRH1

FCRL1 (Fc Receptor-Like Protein 1), also known as CD307a, FcRH1 and IFGP1, is a 36 kDa (predicted) member of the Ig Superfamily. In mouse, it is found on mature follicular and marginal B cells, splenic T cells and NK cells. It would appear that mouse FCRL1 serves as an inhibiting coreceptor, in as much as it contains a series of cytoplasmic ITIM motifs. Mature mouse FCRL1 is a 327 amino acid (aa) type I transmembrane protein (aa 17-343). It contains a 203 aa extracellular region (aa 17‑219) that shows two C2-type Ig-like domains (aa 17-200), and a 103 aa cytoplasmic domain. There are multiple potential splice variants. One shows a deletion of aa 206-248 (potentially soluble), a second contains a six aa substitution for aa 319-343, a third possesses a three aa substitution for aa 209-343, and a fourth shows a 21 aa substitution for Ala11. Over aa 17-219, mouse FCRL1 shares 83% aa sequence identity with rat FCRL1. Over the same region, it shares 63% aa sequence identity with human FCRL1 when a third Ig-like domain that is present in human is excluded from the analysis.

  • Long Name:
    Fc Receptor-like 1
  • Entrez Gene IDs:
    115350 (Human); 229499 (Mouse); 680665 (Rat)
  • Alternate Names:
    BXMH1; CD307a antigen; CD307a; Fc receptor homolog 1; Fc receptor-like 1; FcRH1; FCRH1DKFZp667O1421; FcRL1; FcR-like protein 1; hIFGP1; IFGP family protein 1; IFGP1; IFGP1Fc receptor-like protein 1; Immune receptor translocation-associated protein 5; IRTA5; IRTA5immunoglobulin superfamily Fc receptor, gp42
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 PE-conjugated Antibody

Ctrl IC005P  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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