Mouse Galectin-9 Alexa Fluor® 405-conjugated Antibody

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IC3535V-100UG
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Mouse Galectin-9 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Galectin-9 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Galectin-9 or recombinant mouse Galectin-4 is observed.
Source
Monoclonal Rat IgG2b Clone # 766428
Immunogen
E. coli-derived recombinant mouse Galectin-9 (short isoform)
Ala2-Thr322
Accession # O08573-2
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Mouse thymocytes fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-9

Mouse Galectin-9 ( beta -galactoside-binding lectin 9) is a 36-39 kDa, secreted, S-type lectin. It is 353 amino acids in length and contains no identifiable signal sequence. There are two distinct carbohydrate-binding regions (aa 81-87 and 285-291) that are joined by a linker region (aa 148-205). At least one alternate splice form exists that shows a 31 aa insertion between aa 147-148. The short form is expressed on fibroblasts, hepatocytes, endothelial cells and astrocytes. The long form is expressed on intestinal epithelium. Galectin-9 binds TIM-3 on Th1 cells, inducing apoptosis. Mouse Galectin-9 is 69% and 85% aa identical to human and rat Galectin-9, respectively.

Entrez Gene IDs
3965 (Human); 16859 (Mouse)
Alternate Names
Ecalectin; GAL9; gal-9; galectin 9; Galectin9; Galectin-9; HUAT; lectin, galactoside-binding, soluble, 9; LGALS9; LGALS9A; MGC117375; MGC125973; MGC125974; Tumor antigen HOM-HD-21; urate transporter/channel protein

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